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一个染色体层次的基因组组合的芝麻结论

Hongran Li¹, Yan Peng¹, Chao Wu¹

¹Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518116, P. R. China.

Scientific data

|January 25, 2024

概括

由于气候变化,粉红色的干钻者Sesamia inferens正在扩大其范围. 这项研究提供了高质量的基因组组件,以了解其遗传适应和开发控制策略.

科学领域:

基因组学就是基因组学.
昆虫学昆虫学是一门学科.
农业科学农业科学

背景情况:

粉红色干 (Sesamia inferens) 是一个主要的害虫,其分布向北扩大.
气候变化和改变的农业做法正在推动这种范围的扩大.
了解Sesamia inferens适应的遗传基础对于害虫管理至关重要.

研究的目的:

为了产生高质量的基因组组装Sesamia inferens.
为研究当地适应和开发控制策略提供基因组资源.

主要方法:

使用PacBio和Hi-C技术进行基因组测序.
汇集和注释芝麻的基因组. 推断芝麻.
使用Hi-C数据进行染色体水平的支架.

主要成果:

一个865.04 Mb的基因组组合与一个1.23 Mb的N50结合.
高基因完整性 (96.1%的BUSCO覆盖率).
鉴定了53.14%的重复序列和20858个编码蛋白质的基因.
一个染色体层次的组件,固定在31个染色体上,支架N50为29.99Mb.

结论:

产生的高质量的基因组组合是芝麻菌推断研究的宝贵资源.
这种资源将有助于探索对新环境的遗传适应.
它将支持开发有效的害虫控制策略.

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Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.