Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K
Next-generation Sequencing03:00

Next-generation Sequencing

88.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

On using clustering statistics for assessing plasmid binning tools accuracy.

Briefings in bioinformatics·2026
Same author

Undesignable motifs in structural RNAs and combinatorial consequences.

Journal of mathematical biology·2026
Same author

Genomes from 117 vertebrate species reveal rapidly evolving segmental duplication landscapes.

bioRxiv : the preprint server for biology·2025
Same author

FGF12 Enhances Prostate Cancer Cell Survival via the YB1-lncRNA Axis.

Cells·2025
Same author

AI-driven 3D assessment for Peyronie's disease.

The journal of sexual medicine·2025
Same author

A vector representation for phylogenetic trees.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2025

相关实验视频

Updated: Jul 4, 2025

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.3K

TKSM:高度模块化,用户可定制和可扩展的转录组序列长读模拟器.

Fatih Karaoğlanoğlu1, Baraa Orabi2, Ryan Flannigan3,4

  • 1Computing Science Department, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.

Bioinformatics (Oxford, England)
|January 26, 2024
PubMed
概括

TKSM是一个新的,模块化的长读 (LR) 测序模拟器,可以准确地模拟RNA修改步骤. 这种开源工具通过为基准测试测序工具提供可定制的模拟管道来增强转录组分析.

更多相关视频

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
14:49

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Published on: October 27, 2011

39.2K
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.8K

相关实验视频

Last Updated: Jul 4, 2025

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.3K
Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
14:49

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Published on: October 27, 2011

39.2K
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.8K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • 长读 (LR) 测序提供了具有成本效益的RNA分析,但缺乏用于工具基准测试的黄金标准数据集.
  • 现有的LR模拟器不能完全捕捉像PCR这样的关键库准备步骤,并且很难适应新技术,如单细胞LR测序.

研究的目的:

  • 为长读 (LR) 测序数据开发一个灵活和可扩展的模拟器.
  • 通过结合各种RNA修改步骤的模块化来解决现有模拟器的局限性.

主要方法:

  • TKSM采用模块化设计,每个RNA修改步骤都由专门的模块处理.
  • 模拟器使用核心模块的标准输入/输出格式 (分子描述格式).
  • 用户可以通过结合TKSM模块来组装定制模拟管道,以模拟特定的测序设计.

主要成果:

  • TKSM提供了一个模块化和可扩展的平台,用于模拟长读 (LR) 测序数据.
  • 模拟器明确模拟了各种RNA修改步骤,提高了模拟的准确性.
  • 它的设计使其易于扩展,用于新兴图书馆准备技术的新模块.

结论:

  • TKSM提供了一种灵活和可扩展的解决方案,用于模拟长读 (LR) 测序数据.
  • 模块化方法允许精确模拟各种测序协议和库准备步骤.
  • 这个开源模拟器将有助于开发和对转录组分析工具的基准测试.