开发一种无标签的植物性干扰素玛生产系统,可以提高对病毒的治疗疗效
Min-Chao Jiang1, Wei-Li Hsu2, Ching-Yu Tseng2
1PhD Program in Microbial Genomics, National Chung Hsing University and Academia Sinica, Taichung, Taiwan.
Frontiers in bioengineering and biotechnology
|January 26, 2024
概括
这项研究开发了一种基于植物的表达系统,以产生无标签的治疗性甘氨酸蛋白,如干扰素,具有增强的抗病毒功效. 该系统有效地删除标签,为生物医学应用提供了具有成本效益的替代方案.
科学领域:
- 植物分子生物学 植物分子生物学
- 生物技术是生物技术.
- 病毒学 病毒学
背景情况:
- 植物是生产治疗性葡萄糖蛋白的成本高效平台.
- 上一个基于竹马赛克病毒 (BaMV) 的系统增强了葡萄糖蛋白的产量,但需要标签.
- 融合标签对于重组糖蛋白的生物医学应用是不理想的.
研究的目的:
- 开发一种精细的植物表达系统,用于生产无标签的葡萄糖蛋白.
- 为了增强成熟的干扰素 (mIFNγ) 对病毒的疗效.
- 为了创建一个多功能系统,用于各种目标蛋白的可切割链接器.
主要方法:
- 更新的基于BaMV的表达系统,具有酶或化学可切割的链接器.
- 使用的烟草蚀刻病毒 (TEV) 蛋白酶用于特定的融合蛋白的分裂.
- 采用Ni2+-NTA染色学进行标签和蛋白酶去除.
- 使用Sindbis病毒进行了抗病毒测试.
主要成果:
- 使用TEV蛋白酶分裂部位有效地生产无标签的mIFNγ糖蛋白.
- 通过Ni2+-NTA染色学成功去除标签和蛋白酶.
- 无标签的mIFNγ糖蛋白对Sindbis病毒表现出增强的抗病毒活性.
- 抗病毒活性与商业人类干扰素玛 (hIFNγ) 相当.
结论:
- 改进的基于BaMV的系统有效地产生无标签的治疗性葡萄糖蛋白.
- 该系统为生物医学应用生产糖蛋白提供了一个可行的策略.
- 无标签的mIFNγ糖蛋白表现出强大的抗病毒特性.
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