为什么乳酸细菌在链延长微生物组中壮成长?
Barbara Ulčar1,2, Alberte Regueira1,2,3, Maja Podojsteršek1,2
1Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Ghent University, Gent, Belgium.
Frontiers in bioengineering and biotechnology
|January 26, 2024
概括
这项研究描述了废物到化学生物工艺中的乳酸细菌和链延长细菌. 了解它们的生长动态是优化从有机废物中生产酸的关键.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 环境科学与工程环境科学与工程
- 微生物学和微生物生态学
背景情况:
- 有效的废物管理对于一个可持续的社会至关重要.
- 混合培养生物技术为从有机废物中生产有价值的化学品提供了一个有前途的途径.
- 基于知识的生物工艺开发需要详细了解微生物社区的相互作用和生长特征.
研究的目的:
- 通过以糖为基础的链延长建立一个颗粒性污泥生物工艺,用于生产酸.
- 描述关键微生物协会的生长特征,特别是乳酸细菌和链延长细菌.
- 了解影响生物过程效率的代谢相互作用和生长策略.
主要方法:
- 建立了利用基于糖的链延长代谢的颗粒性污泥生物工艺.
- 隔离和表征了乳酸细菌 (Limosilactobacillus musocae G03) 和链延长细菌 (Caproiciproducens lactatifermentans) 的代表菌株.
- 量化了生长速度,生物质产量,半和常数,并确定了酸对微生物生长的抑制作用.
主要成果:
- 乳酸细菌的生长率较高 (0.051小时−1),但生物质产量较低 (0.120克/克葡萄糖),与链延长细菌 (0.026小时−1,0.239克/克葡萄糖) 相比,表明了明显的r-和K-战略行为.
- 在L. musocae中葡萄糖的半和常数为0.35g/L.
- 对于L. musocae,确定了高卡酸抑制度13.6g/L,在暴露前恢复低度的生长率.
结论:
- 乳酸细菌和链延长细菌的不同生长策略显著影响基于糖的链延长系统.
- 乳酸细菌的高酸耐受性对于它们在酸生产系统中的持久性和功能至关重要.
- 这些发现为优化微生物社区相互作用和设计更高效的生物工艺从废物中生产化学品提供了关键的见解.
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