一个集成的ddPCR实验室盘上装置,用于快速查传染病
Wanyi Zhang1, Lili Cui2,3, Yuye Wang4
1Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR 999077, China.
Biosensors
|January 26, 2024
概括
一个便携式实验室在磁盘上的设备可以快速地在现场使用数字滴滴PCR (ddPCR) 进行病毒核酸量化. 这个集成系统执行滴滴生成,热循环和分析,为传染病查提供灵活的工具.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 医学诊断 医学诊断 医学诊断
背景情况:
- 数字滴滴PCR (ddPCR) 提供了精确的病毒核酸量化,但缺乏可移植性的现场查.
- 目前商业的ddPCR系统只能在实验室内使用,这阻碍了紧急临床需求的快速部署.
研究的目的:
- 开发一款便携式,完全集成的实验室在磁盘 (LOAD) 设备,用于定量,现场的传染病查.
- 为了证明该设备对准确的病毒定量和临床样本分析的能力.
主要方法:
- 在一个紧的LOAD设备中整合了微流体,循环的基于油的换热器和磁盘上的光成像.
- 使用10^7稀释范围内的A型流感病毒DNA片段进行验证.
- 使用含有五种不同病毒 (IAV,RSV,VZV,ZIKV,ADV) 的临床样本进行测试.
主要成果:
- 通过DNA度和稀释因子之间的线性相关性来验证精确的量化.
- 检测临床样本的最低度为20.24副本/μL.
- 在使用集成磁盘成像系统的临床样本中成功检测了流感A病毒的概念验证.
结论:
- 集成的ddPCR-LOAD设备为定量传染病查提供了一个便携式和高效的解决方案.
- 这项技术有可能成为现场临床诊断的灵活工具.
- 该设备在临床环境中检测各种病毒病原体方面表现出强大的性能.
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