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Ana María Acevedo¹, Lydie Postic², Maray Curiel¹

¹National Center for Animal and Plant Health (CENSA), Carretera de Tapaste y Autopista Nacional, Apartado Postal 10, San José de las Lajas, San José de las Lajas 32700, Cuba.

|January 26, 2024

概括

在古巴确认了蓝舌病病毒 (BTV) 的传播,在牛和绵羊中确定了12种不同的血清型. 这强调了需要加强对牲畜和昆虫载体中BTV的监测.

科学领域:

兽医病毒学兽医病毒学
分子流行病学分子流行病学
关节动物传播的病毒

背景情况:

蓝舌病病毒 (BTV) 在全球范围内对牲畜构成重大威胁.
古巴的BTV血清流行率很高,但循环中的血清型仍未确定.
了解BTV多样性对于有效的疾病管理至关重要.

研究的目的:

为了识别在古巴西部循环的BTV血清型.
调查牛和绵羊种群中的BTV流行情况.

主要方法:

病毒学分析包括PCR,病毒隔离和病毒中和.
使用RT-PCR和序列独立单一原始放大 (SISPA) 的BTV分离物的基因组测序.
MinION 牛津纳米孔测序技术可实现全基因组的恢复.

主要成果:

在年轻牛和绵羊中确认了BTV循环,病毒发病率为25%.
从18个血液样本中分离出BTV.
通过基因组测序确定了十二种BTV血清型 (BTV-1, 2, 3, 6, 10, 12, 13, 17, 18, 19, 22, 24).

结论:

在一个受限制的古巴地区,证明了各种各样的BTV血清型的共同循环.
强调迫切需要对BTV进行综合昆虫学和畜牧业监测.
强调监测BTV的重要性,因为全球分布和感染模式正在发生变化.

关键词:

这是BTV的BTV.纳米孔测序的测序这是一个PCRPCR.桑格尔的桑格尔的隔离隔离隔离隔离隔离血清型类型血清型类型

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