用脂质体多克索鲁比辛和诱导性中度高温症对肉瘤Saos-2细胞的联合治疗
Valerii E Orel1,2, Anatoliy G Diedkov1, Vasyl V Ostafiichuk1
1National Cancer Institute, 33/43 Zdanovska Str., 03022 Kyiv, Ukraine.
Pharmaceuticals (Basel, Switzerland)
|January 26, 2024
概括
诱导性中度高温症 (IMH) 增强了脂质体内多克索鲁比 (LDOX) 输送和骨髓瘤细胞中的抗瘤作用. 这种组合疗法增加了细胞死亡和反应性氧物种的产生,这表明了体内研究的潜力.
科学领域:
- 生物医学工程 生物医学工程
- 在瘤学瘤学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 骨髓瘤 (OS) 仍然是一个具有挑战性的癌症,正在研究新的治疗策略.
- 在OS治疗中,脂质体 doxorubicin 配方 (LDOX) 的疗效可能受到药物输送和细胞耐药性的限制.
- 诱导性中度高温症 (IMH) 在增强LDOX抗瘤活性中的作用需要进一步阐明.
研究的目的:
- 为了研究LDOX和IMH对Saos-2人类骨髓瘤细胞的联合作用.
- 评估IMH对LDOX输送,细胞活力,细胞亡和反应性氧物种 (ROS) 生成的影响.
- 评估在组合治疗下对亲细胞亡性巴克斯蛋白表达的调节.
主要方法:
- 用LDOX,IMH (42MHz,15W,30分钟) 或两者的组合对待Saos-2细胞.
- 细胞活力使用试蓝色试验进行了评估.
- 用流式细胞计量测量了亡和ROS产生.
- 通过免疫细胞化学检查巴克斯蛋白表达.
主要成果:
- 与未经治疗的对照组相比,组合疗法显著降低了61%的Saos-2细胞活力.
- IMH增强了LDOX的有效性,导致在低于42°C的温度下增加ROS的产生.
- 在LDOX加IMH治疗 (p <0.05) 时,观察到早期亡的12%增加和Bax异质性的减少.
结论:
- IMH增强了骨髓瘤细胞中的LDOX输送和抗瘤作用.
- 组合疗法机制涉及改变的膜通透性和增加的ROS生成.
- 研究结果表明,IMH与LDOX相结合,有可能成为未来的体内骨髓瘤治疗策略.
关键词:
巴克斯蛋白质是巴克斯的蛋白质.在Saos-2细胞中.它具有抗瘤效果的作用.结合治疗治疗的组合治疗.早期的细胞灭亡 (apoptosis)诱导性中度高温症 诱导性中度高温症脂质体 doxorubicin 的使用情况.骨质肉瘤是骨质肉瘤的一种疾病.有活性氧物种的反应性氧物种.瘤细胞的异质性更多相关视频
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