黑色素重塑蛋白ATRX在老鼠早期胚胎中的核分布在正常发育和发育停滞期间在体外
Irina O Bogolyubova1, Zhuldyz K Sailau2, Dmitry S Bogolyubov1
1Institute of Cytology of the Russian Academy of Sciences, St. Petersburg 194064, Russia.
Life (Basel, Switzerland)
|January 26, 2024
概括
染色体重塑蛋白ATRX对于早期哺乳动物发育至关重要. 它在小鼠胚胎中的核分布表明了发育的可行性,特别是当它与周心DNA相关时.
科学领域:
- 发展生物学 发展生物学
- 遗传学和基因组学 遗传学和基因组学
- 细胞生物学 细胞生物学
背景情况:
- 依赖ATP的染色体旋酶转位酶ATRX对于基因组稳定性至关重要,在哺乳动物的 oogenesis 和胚胎发生中发挥着至关重要的作用.
- 在关键的发育阶段,ATRX作为基因组护理者,维护基因完整性.
研究的目的:
- 为了研究ATRX蛋白在小鼠胚胎中的内核分布在体内和体外发育过程中.
- 确定ATRX定位,周心DNA,核活性和胚胎活力之间的关系,特别是在两细胞块阶段.
主要方法:
- 免疫光标签和光现场混合 (FISH) 被用于可视化小鼠胚胎中的ATRX分布.
- 分析了不同发育阶段的胚胎,包括正常发育的胚胎和体外培养胚胎,经历了双细胞阻断.
- 核动素在ATRX协会中的作用被使用拉特伦库林B治疗来评估.
主要成果:
- 在正常发育的胚胎中,ATRX与围心体DNA结合在一起,这是一种依赖于核活性的关联.
- 拉特伦库林B的治疗破坏了ATRX-pericentromeric异色染色素的关联.
- 在体外培养的胚胎,尤其是双细胞区块下的胚胎,显示出改变的ATRX分布,与主要卫星DNA的关联性减少.
结论:
- ATRX的核内分布模式与小鼠胚胎的生存能力密切相关.
- 在ATRX局部的变化,特别是它与周周中心基异色素的分离,可以预测成功的植入前发育.
- 核活性动力学影响ATRX定位和潜在的胚胎发育能力.
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