与C6质瘤细胞不同的是,星细胞中的S100B分泌被乳酸盐下调
Nicholas Guerini Selistre1, Leticia Rodrigues1, Barbara Carolina Federhen1
1Biochemistry Post-Graduate Program, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre 90010-150, Brazil.
Metabolites
|January 26, 2024
概括
酸 (FC) 在星球细胞和C6质瘤细胞中不同影响S100B分泌. 细胞外乳酸介导物FC 细胞外乳酸介导物
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- S100B是一种由天体细胞分泌的结合蛋白.
- C6质瘤细胞用于模拟星球细胞,但在高葡萄糖中显示不同的S100B分泌.
- 酸 (FC) 是克雷布斯循环抑制剂,影响S100B分泌,这表明它与新陈代谢有关.
研究的目的:
- 研究FC对S100B分泌在初级星球细胞,海马片和C6质瘤细胞中的影响.
- 探索乳酸在调解这些效应中的作用.
- 了解原始细胞和C6质瘤细胞之间S100B代谢调节的差异.
主要方法:
- 用FC治疗原发性天体细胞培养,急性海马切片和C6质瘤细胞.
- 对S100B分泌物的分析.
- 对细胞外乳酸水平的研究.
- 用乳酸及其激动剂 (切片和C6细胞) 治疗.
- 在C6细胞中评估HCAR1表达.
主要成果:
- 在初级星球细胞和海马切片中,FC降低了S100B分泌,但不是C6细胞.
- 在这两种模型中,FC反向改变了细胞外乳酸水平.
- 乳酸盐治疗降低了切片中的S100B分泌量,但没有降低C6细胞的分泌量,尽管HCAR1的表达.
- 与星球细胞相比,C6质瘤细胞对S100B的代谢调节有明显的表现.
结论:
- 细胞外乳酸调节了FC治疗的星体细胞中S100B分泌量的减少.
- C6质瘤细胞表现出独特的能量代谢途径.
- HCAR1通路为S100B和葡萄糖代谢之间的联系提供了证据,突出了细胞特异性的差异.
更多相关视频
15:08Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions
Published on: October 21, 2017
9.9K
10:28Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells
Published on: January 10, 2018
8.3K
相关概念视频
02:17The Tumor Microenvironment
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
02:40Cancer Stem Cells and Tumor Maintenance
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
01:19Cadherins in Tissue Organization
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...