在 (预处理) 酵母中对微封装进行了定量重新评估
Giulia Coradello1,2, Chiara Setti1, Roberto Donno1
1Laboratory for Polymers and Biomaterials, Fondazione Istituto Italiano di Tecnologia, 16163 Genova, Italy.
Molecules (Basel, Switzerland)
|January 26, 2024
概括
酵母细胞对疏水化合物的封装受酵母预处理和疏水性质的影响. 未经处理的酵母和溶剂辅助工艺提供了更好的封装效率和保护具有挑战性的恐水虫.
科学领域:
- 生物技术是生物技术.
- 生物材料科学 生物材料科学
- 食品科学 食品科学 食品科学
背景情况:
- 疏水化合物被封装在酵母细胞中以保护和减少蒸发.
- 工业对酵母封装的兴趣很高,但关键参数仍然不清楚.
- 了解酵母预处理,水性极性和工艺条件至关重要.
研究的目的:
- 调查酵母预处理,水极性和工艺条件如何影响封装效率和保护.
- 开发分析方案,以评估这些对*Saccharomyces cerevisiae*的影响.
- 为了优化各种疏水化合物的酵母封装过程.
主要方法:
- 使用的*Saccharomyces cerevisiae*经过各种预处理 (未经处理,以乙醇提取,NaCl-plasmolyzed,细胞壁枯竭).
- 使用三种工艺类型 (纯油,水分散,乙溶液) 测试了三种不同极性的水剂 (linalool, 1,6-dihydrocarvone, limonene).
- 通过使用开发的分析协议和主要成分分析,评估酵母形态,透性和封装效率.
主要成果:
- 更严厉的酵母预处理增加了水性扩散,但降低了封装效率和保护.
- 更多的疏水性烯在纯油或水分散中表现出较低的封装,但在乙中差异较小.
- 溶剂/分散剂,如乙或二次水剂,显著改善了具有挑战性的化合物的封装.
结论:
- 未经处理的酵母通常为封装工艺提供优势.
- 基于溶剂或辅助二次疏水的工艺对于封装难以处理的疏水化合物是有益的.
- 优化酵母封装需要仔细考虑酵母制备和加工方法,以适应特定的厌水生物.
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