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红色激光激活的银纳米颗粒与葡萄提取物合成的抗菌作用,可以对抗金黄色葡萄球菌和大肠杆菌

Ahmad Khalil Yaqubi¹, Suryani Dyah Astuti², Andi Hamim Zaidan¹

¹Department of Physics, Faculty of Science and Technology, Universitas Airlangga, Surabaya, 60115, Indonesia.

Lasers in medical science

|January 26, 2024

概括

这项研究表明,将葡萄种子提取物衍生的银纳米粒子 (AgNPs-GSE) 与405nm红色激光相结合,可以有效地使大肠杆菌和金黄色细菌失活. 治疗显著降低了细菌活力,提供了一个有前途的抗菌方法.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
微生物学微生物学

背景情况:

像细菌这样的微生物对健康构成重大风险,需要有效的抗微生物战略.
银纳米粒子 (AgNPs) 以其抗菌和抗病毒特性而闻名,使其在生物医学应用中具有价值.
葡萄种子提取物作为合成银纳米粒子 (AgNPs-GSE) 的天然来源.

研究的目的:

为了评估红色激光和AgNPs-GSE在光灭活格兰阴性大肠杆菌和格兰阳性黄金葡萄球菌的疗效.
为了确定最佳的度和辐射时间,以有效的细菌失活.
评估AgNPs-GSE和激光治疗对细菌活力的联合作用.

主要方法:

细菌样本 (大肠杆菌和黄金杆菌) 被分为对照组和实验组.
实验组接受了AgNPs-GSE (光敏感剂) 治疗,并用405nm二极管激光照射,时间和度各不相同.
细菌培养被化,照射,在TSA介质上培养,并使用殖民地计数和统计测试 (ANOVA,Tukey) 进行分析.

主要成果:

10μl的AgNPs-GSE,405nm红色激光和3.44 J/cm2能量密度的组合有效地使大肠杆菌和金黄色杆菌的光不活化.
对于大肠杆菌来说,在2mM AgNPs-GSE和180s辐射下,细菌活力降低了高达79.53%.
对于S. aureus,在2mM的AgNPs-GSE和180s辐射下,细菌活力降低了85.04%,高达85.04%.

结论:

AgNPs-GSE和红色激光照射的协同效应为光灭活大肠杆菌和金黄色杆菌提供了一种强有力的方法.
这种方法显示出开发新型抗菌疗法的巨大潜力.
该研究强调了基于纳米技术的解决方案在对抗细菌感染方面的有效性.

关键词:

抗菌剂抗菌剂抗菌剂葡萄种子提取物葡萄种子提取物绿色合成是一种绿色合成.激光疗法是一种激光疗法.银纳米颗粒银纳米粒子

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