经常性病毒捕获细胞固酶,这些细胞抗OAS-RNase L
Stephen A Goldstein1,2, Nels C Elde1,2
1Department of Human Genetics, University of Utah, School of Medicine, Salt Lake City, UT 84112.
概括
病毒化酶 (PDEs) 通过横向基因转移获得,抑制先天免疫力. 我们的研究揭示了至少五个独立的收购和复杂的进化历史,这些病毒PDEs.
科学领域:
- 病毒学 病毒学
- 进化生物学 进化生物学
- 免疫学 免疫学 免疫学
背景情况:
- 病毒化酶 (PDEs) 对于抑制OAS-RNase L抗病毒通路至关重要.
- 病毒性PDEs的进化起源和获取事件在很大程度上仍然不清楚.
- 了解病毒性PDE的起源是理解病毒性免疫逃避策略的关键.
研究的目的:
- 调查病毒固酶 (PDEs) 的进化史和起源.
- 识别病毒之间的独立基因获取事件和水平基因转移实例.
- 探索病毒PDEs的序列和结构差异之间的关系.
主要方法:
- 遗传学分析以追踪PDE基因获取事件.
- 保存模式分析以确定祖先联系.
- 祖先序列重建和结构建模以评估蛋白质分歧.
主要成果:
- 在不同病毒类型中确定了至少五个独立的PDE获取事件.
- 提供了冠状病毒之间PDE基因水平基因转移的证据.
- 揭示了复杂的进化模式,其中序列和结构分歧并不总是相关的.
- 突出显示了Nidovirales (merbecoviruses, embecoviruses, toroviruses) 和罗塔病毒中独立的PDE获取.
结论:
- 病毒性PDEs具有复杂和动态的进化历史,涉及多个独立的收购和水平基因转移.
- 这项研究为未来对病毒PDE演变和功能的研究提供了框架.
- 尽管其序列保存率低,但病毒性PDEs与哺乳动物AKAP7蛋白质有共同的祖先.
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