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基因组下一代测序在患有不同器官系统感染的患者中的临床应用:一项追溯观察性研究

Haiyan Zhang¹, Fengli Zhou, Xiaoyun Liu

¹Department of General Practice, The Third Affiliated Hospital of Sun Yat-Sen University Lingnan Hospital, Guangzhou, Guangdong Province, People's Republic of China.

|January 26, 2024

概括

与传统方法相比,甲基因组下一代测序 (mNGS) 在传染病中检测病原体的灵敏度更高. 虽然mNGS在识别多样化和罕见的病原体方面表现出色,但它对传统技术的优势因病原体类型而异.

科学领域:

临床微生物学临床微生物学
传染病诊断传染病诊断传染病诊断
下一代测序应用程序的下一代测序应用程序.

背景情况:

传统的微生物识别方法往往很慢,缺乏灵敏度.
甲基因组下一代测序 (mNGS) 是一种无培养,无假设的诊断方法.
mNGS有潜力检测广泛的病原体.

研究的目的:

评估mNGS在各种器官系统中的诊断实用性,用于传染病.
将mNGS的灵敏度和特异性与传统诊断方法进行比较.

主要方法:

回顾性队列研究,包括94名患有mNGS的患者.
将mNGS阳性率与传统方法 (涂抹,血清学,PCR,培养) 的比较.
诊断性能指标的分析,包括积极和消极的预测值.

主要成果:

mNGS在54.26%的培养结果为阴性的情况下识别了病原体.
与培养 (P < .001) 相比,mNGS显示敏感度显著增加 (78.05%与24.39%).
mNGS比传统的病原体识别方法具有更高的特异性,特别是对于新兴或罕见的病原体.

结论:

mNGS显著提高了传染病的病原体检测灵敏度,特别是细菌和整体病原体识别.
mNGS对于识别新兴或罕见的病原体很有价值,有助于治疗方案的指导.
mNGS的诊断优势各不相同,与常规的真菌,病毒或结核病检测方法相比没有明显的优势.

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