两种用于X射线成像和数字体积相关性测量在软骨骨接口的两种增强对比度的染色剂的比较
Sarah Davis1, Aikaterina Karali2, Tim Balcaen3
1School of Pharmacy and Biomedical Science, University of Portsmouth, Portsmouth, PO1 2DT, UK; School of Mechanical and Design Engineering, University of Portsmouth, PO1 3DJ, UK.
Journal of the mechanical behavior of biomedical materials
|January 26, 2024
概括
用哈夫替代的威尔斯-道森聚氧甲 (Hf-WD POM) 在骨关节炎研究中的软骨结构可视化方面优于酸 (PTA). 在成像过程中,Hf-WD POM保留了组织的机械特性,与PTA不同,PTA显著改变了刚性.
科学领域:
- 生物机械工程 生物机械工程
- 材料科学 材料科学 材料科学
- 骨关节炎研究 骨关节炎研究
背景情况:
- 骨关节炎 (OA) 的发病过程涉及到影响软骨机械的亚冠骨变化.
- 了解软骨-骨接口的机械相互作用对于OA研究至关重要.
- 需要高分辨率的成像技术来评估软骨中菌株的分布.
研究的目的:
- 为了比较两种增强对比度的染色剂 (CESA):酸 (PTA) 和替代的威尔斯-道森聚氧甲 (Hf-WD POM).
- 评估它们适用于将高分辨率对比度增强的X射线微焦计算机断层扫描 (CECT) 与数字体积相关 (DVC) 结合起来,用于全场应变测量.
- 评估这些剂对软骨机械性质的影响.
主要方法:
- 牛骨质支柱塞被PTA或Hf-WD POM染色.
- 机械性能使用微机械探测和纳米冲刺进行了评估.
- 在CECT成像后进行无限制的压缩测试后计算了残留菌株.
主要成果:
- 无论是PTA还是Hf-WD POM,都能够可视化软骨结构以进行DVC分析.
- PTA染色显著增加了软骨的刚性,减少了模量和硬度.
- 用Hf-WD POM染色的标本保留了与染色前组织相似的机械性能,硬度仅略有增加.
结论:
- 在OA研究中,Hf-WD POM是一个更适合用于CET-DVC分析的CESA.
- Hf-WD POM允许准确的应变测量,同时保持本地软骨的机械性能.
- PTA显著改变了软骨的生物力学,限制了其在机械研究中的使用.
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