对10-50μm浮游生物的腺三酸盐 (ATP) 模型的验证
Louis Peperzak1, Oscar Casas-Monroy2, Sarah A Bailey2
1NIOZ Royal Institute for Sea Research, Department of Estuarine & Delta Systems, PO Box 59, NL-1790 AB Den Burg, the Netherlands.
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|January 26, 2024
概括
这项研究验证了水生生物中腺三酸盐 (ATP) 含量的模型. 经过验证的ATP模型准确地估计了10-50微米大小范围内的生物体的生物质.
科学领域:
- 环境科学 环境科学
- 海洋生物学 海洋生物学
- 水生生态学 水生生态学
背景情况:
- 一个模型先前估计了球形水生生物 (10-50微米直径) 的腺三酸盐 (ATP) 含量在0.1619.9 pg cell-1.1.
- 准确的生物质估计对于生态研究至关重要,包括压载水管理.
研究的目的:
- 为了验证水生生物中ATP含量的预测模型.
- 为了比较显微镜计数与使用商业套件测量的ATP度.
- 评估模型对10-50微米大小范围内的生物的可靠性.
主要方法:
- 显微镜被用于细胞计数.
- 用商业ATP套件测量ATP度.使用商业ATP套件测量ATP度.
- 从淡水和海洋生物 (10-50μm) 收集和分析了数据.
主要成果:
- 淡水和海洋生物 (10-50微米) 的测量ATP含量处于模型预测范围 (0.1619.9 pg cell-1).
- 淡水生物的平均含量为0.33ppg的ATP细胞-1,其球体直径相当于13μm.
- 海洋生物的平均含量为0.89ppg的ATP细胞-1,SED为18微米.
结论:
- ATP 含量模型可靠地估计在指定尺寸范围内的水生生物中的生物量.
- 在将3D生物质代理转换为线性细胞度时,建议谨慎.
- 这些发现支持使用ATP作为水生环境中的生物质指标.
相关概念视频
ATP Driven Pumps I: An Overview
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and are...
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and are...
ATP Synthase: Mechanism
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased ATP...
ATP Synthase: Structure
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
ATP Driven Pumps III: V-type Pumps
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...


