相关实验视频

Updated: Jul 4, 2025

Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series

Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series

Published on: May 10, 2020

在Clostridioides difficile中,鞭毛和毒素的相变是通过选择性Rho-依赖终止介导的

Mercedes A H Warren Norris¹, Dylan M Plaskon¹, Rita Tamayo¹

¹Department of Microbiology and Immunology, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC, USA.

Journal of molecular biology

|January 26, 2024

概括

克洛斯特里迪奥伊德困难Rho选择性地结合并终止.

科学领域:

微生物学微生物学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

困难菌是一种肠道病原体.
在C. difficile的相变控制鞭毛和毒素表达通过鞭毛 (flg) 开关.
转录终止因子Rho通过flg-OFF开关抑制细菌的运动性.

研究的目的:

阐明C. difficile Rho区分flg-ON和flg-OFFmRNA的机制. 为了阐明C. difficile Rho区分flg-ON和flg-OFFmRNA的机制.
确定Rho的N端插入域 (NID) 和选择性终结中的其他域的作用.

主要方法:

试验室ATPase测试以评估Rho与flg开关序列的结合.
在C. difficile体内进行体内和体内测试,以评估Rho功能.
对Rho域的生物化学和遗传分析.

主要成果:

罗选择性地结合了flg-OFF mRNA的左反转重复.
NID对于Rho介导的flg-OFFmRNA终结至关重要,可能通过影响六合体形成.
该RNA结合域对于flg-OFF特定终结至关重要.

结论:

C. difficile Rho采用了一种新的机制,涉及选择性mRNA结合和特定域的功能来调节相变.
这项研究提供了关于AT丰富的细菌基因组中Rho介导终结的见解.

关键词:

鞭毛状切换器的切换器基因调节基因调节基因调节异质性的异质性运动性运动性运动性转录终止转录终止

更多相关视频

Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility

Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility

Published on: March 11, 2022

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Published on: May 30, 2017

相关概念视频

Transcription Attenuation in Prokaryotes

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...

Cell Polarization by Rho Proteins

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...

Termination of Translation

Termination of Translation

The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...