玛辐射对病原性黄金葡萄球菌在用生物提取物处理的包装成食沙拉中的作用
Widad Zernadji1, Sihem Jebri2, Faten Rahmani2
1University of Carthage, Higher School of Food Industries, 1003 Tunis, Tunisia; Laboratoire de Biotechnologies et Technologie Nucléaire, CNSTN, Sidi Thabet 2020, Tunisia.
Journal of food protection
|January 26, 2024
概括
这项研究表明,将玛辐射与西红精油和花果汁相结合,可以有效地在即食沙拉中的金黄色葡萄球菌 (Staphylococcus aureus) 失活. 该处理保持了可接受的感觉质量,为消费者提供了安全的选择.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 食品安全 食品安全
背景情况:
- 准备食用的 (RTE) 沙拉需要对病原体进行控制,特别是对于免疫力低下的人来说.
- 黄金葡萄球菌 (黄金葡萄球菌) (S. aureus) 在RTE食品中是一个重大问题.
- 精油 (EO) 和天然果汁显示出抗菌潜力.
研究的目的:
- 为了研究玛辐射与西红精油 (EO) 和果汁相结合的疗效,在RTE沙拉中对S. aureus进行检测.
- 评估这种联合处理在储存期间对沙拉的感官特性的影响.
- 评估这些干预措施对病原体失活的协同潜力.
主要方法:
- 通过水蒸从头皮 (Thymus capitatus) 提取EO,通过GC/FID和GC-MS进行分析.
- 从新鲜水果中制备树果汁.
- 用玛辐射 (0.5kGy),EO (0.08μL/mL) 和果汁 (6μL/mL) 处理S. aureus注射的沙拉,然后存储10天.
- 通过感觉面板评估黄金色细菌减少 (log CFU/g) 和感官特性.
- 对EO对HUVEC细胞的细胞毒性评估.
主要成果:
- 蒂EO成分分析显示,卡瓦克罗尔 (79.55%) 和p-cymene (7.93%) 是主要成分.
- 在低于0.16μL/mL的度下,EO是非细胞毒性的.
- 用0.5kGy玛辐射,6μL/mL果汁和0.08μL/mL西兰花EO的组合,实现了超过3logCFU/g的减少和S. aureus的完全无活化.
- 感官评估表明,处理过的沙拉是可以接受的.
- 联合治疗在S. aureus无活化中显示出协同效应.
结论:
- 玛辐射,西红EO和果汁的组合在RTE沙拉中的S. aureus无活化方面非常有效.
- 这种多重障碍的方法为提高RTE食品的微生物安全提供了一个有希望的策略.
- 治疗有效地减少病原体,而不会影响沙拉的感官可接受性.
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