阿尔基米西亚 (Artemisia argyi) 提取物分片通过破坏线粒体形态和功能来对皮肤细胞产生抗真菌作用
Le Chen1, Yunyun Zhu2, Chaowei Guo2
1College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China; College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Chinese journal of natural medicines
|January 26, 2024
概括
阿尔基水提取物 (AAWE) 显示出强大的抗真菌活性对皮肤细胞. 活性亚分部分AAWE6破坏了真菌线粒体功能和形态,验证了其传统医疗用途.
科学领域:
- 菌类学 菌类学是指菌类学.
- 药理学是指药理学,即药理学是指药理学.
- 生物化学 生物化学
背景情况:
- 亚特米西亚阿尔吉 (Artemisia argyi,A. argyi) 传统上在亚洲因其消毒性而被使用.
- 对A. argyi水提取物 (AAWE) 对皮肤细胞的抗真菌功效的科学证据有限.
- 这项研究调查了A. argyi对关键皮肤植物物种的抗真菌作用和分子机制.
研究的目的:
- 评估A. argyi水提取物 (AAWE) 对皮肤植物的抗真菌功效.
- 识别活性子部分并阐明其分子作用机制.
- 为A. argyi. 的传统使用提供科学验证.
主要方法:
- 对Trichophyton rubrum,Trichophyton mentagrophytes和Microsporum gypseum进行抗真菌活性测试. 这种真菌有着非常高的抗真菌活性.
- 使用D101宏树脂,分离和识别活性子部分 (AAWE6).
- 传输电子显微镜 (TEM) 用于形态分析.
- 酶活性测试,转录组分析,RT-qPCR和生物化学测试以调查分子机制.
- 化学表征和分子对接以识别活性化合物.
主要成果:
- AAWE证明了对测试的皮肤植物有显著的抗真菌作用.
- 小部分AAWE6表现出强烈的活性,具有特定的最小抑制度 (MIC).
- TEM发现AAWE6破坏了真菌细胞完整性和线粒体形态.
- 分子分析证实了线粒体功能,TCA循环和能量代谢的破坏.
- 黄类化合物,eupatilin和jaceosidin被确定为AAWE6.6中的主要活性成分.
结论:
- 艺术 argyi亚分离AAWE6对皮肤细胞具有强大的抗真菌作用.
- 该机制涉及线粒体形态和功能的破坏.
- 这项研究在科学上证实了A. argyi在抗真菌应用中的传统用途.
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