转录因子AP2XI-2是Toxoplasma gondii merogony的一个关键的负调节器
Jin-Lei Wang1, Ting-Ting Li2, Nian-Zhang Zhang2
1State Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, 730046, People's Republic of China. hany.elsheikha@nottingham.ac.uk.
Nature communications
|January 26, 2024
概括
两种转录因子AP2XI-2和AP2XII-1被发现可以抑制Toxoplasma gondii的性承诺. 它们的枯竭在特定条件下触发了无性繁殖 (美),提供了新的研究模式.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 在 *Toxoplasma gondii* 中的性发育导致卵囊的产生,这是人类感染的关键因素.
- 在*Toxoplasma gondii*中,对性承诺的分子控制尚未得到充分理解.
研究的目的:
- 为了研究*Toxoplasma gondii**中调节性承诺的分子机制.
- 确定参与抑制无性发育期间的性前承诺的关键调节者.
主要方法:
- 研究了转录因子AP2XI-2和AP2XII-1在*Toxoplasma gondii*性发育中的作用.
- 在不同pH条件下 (中性和性) 在II型Pru菌株中利用基因枯竭策略.
- 确定了参与调节 merozoite 编程的 AP2XI-2 相互作用蛋白.
主要成果:
- AP2XI-2和AP2XII-1的功能是性承诺的负调节者.
- 在性,但不是中性的介质中,AP2XI-2的耗尽诱导了美洛和美洛产量.
- 在中性和性条件下,AP2XII-1的耗尽促进了美,在性介质中效果更大.
- 另外两种与AP2XI-2相互作用的蛋白质被确定为 merozoite 发育的抑制剂.
结论:
- 包括AP2XI-2和AP2XII-1在内的一个因素网络,在*Toxoplasma gondii*中复杂地调节了性前承诺.
- AP2XI-2或AP2XII-1-贫的*毒素菌*Pru菌株为体外美研究提供了有价值的模型.
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