皮肤再生和光衰老保护使用由脂肪衍生的干细胞带有外源性载荷的细胞外囊泡
Diem D N Nguyen1, Diem My Vu2, Nhan Vo1
1Medical Genetics Institute, Ho Chi Minh City, Vietnam.
概括
脂肪酸衍生干细胞小细胞外囊泡 (ASC-sEVs) 充满了抗衰老化合物尼古丁胺胺 рибо和白醇. 在人体试验中,这种组合在保护皮肤免受光衰老和改善皮肤再生方面表现出协同作用.
科学领域:
- 生物技术是生物技术.
- 再生医学是一种再生医学.
- 皮肤病学 皮肤病学
背景情况:
- 来自脂肪衍生干细胞 (ASC) 的小细胞外囊 (sEV) 显示出皮肤抗衰老的前景.
- 在ASC-sEV中封装天然抗衰老化合物以获得协同效应尚未得到充分研究.
- 对于工业应用,需要标准化,具有成本效益的方法来生产纯ASC-sEV.
研究的目的:
- 开发一种标准化的协议,用于生产高纯度的ASC-sEV.
- 研究装载ASC-sEV与尼古丁胺二 (NR) 和复星的协同抗衰老效应.
- 评估装载ASC-sEVs在保护UVB诱导的皮肤损伤和皮肤再生方面的有效性.
主要方法:
- 在无血清介质中优化ASC培养,用于sEV生产.
- 纯化的ASC-sEV使用触流过和超离心,然后进行全面的表征 (蛋白质组学,脂组学,miRNA测序).
- 用NR和resveratrol装载的ASC-sEV通过超声波化;在暴露于UVB的HaCaT细胞和人类志愿者皮肤试验中测试了有效性.
主要成果:
- 一个强大的工作流产生了高纯度的ASC-sEV,具有一致的特性和货物.
- 负载的ASC-sEV显著减少了UVB暴露的角质细胞中的活性氧物种 (ROS),超过了单个组件的性能.
- 人类试验显示,加载ASC-sEV可以改善皮肤质地,增加水分和弹性104%,并在8周内减少毛孔体积51%.
结论:
- 建立了一个可靠的生产和装载ASC-sEV与天然化合物的协议.
- 装载的ASC-sEV显示出协同的抗衰老特性,结合了sEV和封装化合物的好处.
- 这种方法有可能用于针对光衰老和皮肤再生的先进护肤配方.
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