相关实验视频

Updated: Jul 4, 2025

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

通过SARS-CoV-1和SARS-CoV-2编码的circRNA的识别,特征和表达分析

Mengting Niu^1,2, Chunyu Wang³, Yaojia Chen^4,5

¹School of Electronic and Communication Engineering, Shenzhen Polytechnic University, Shenzhen 518055, China.

Briefings in bioinformatics

|January 27, 2024

概括

这项研究表明,SARS-CoV-1和SARS-CoV-2编码圆形RNA (circRNAs),而SARS-CoV-2显示出更大的能力. 这些病毒circRNA与癌症有关,可能调节宿主细胞功能.

科学领域:

病毒学病毒学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

病毒编码的循环RNAs (circRNAs) 在免疫反应中起作用,可以作为治疗点或生物标志物.
冠状病毒SARS-CoV-1和SARS-CoV-2 (SARS-CoV-1/2) 具有高度传染性和致命性,但它们编码的circRNAs在很大程度上仍未表征.

研究的目的:

为了调查SARS-CoV-1/2是否对circRNAs进行编码.
描述这些病毒circRNA的特征和功能.

主要方法:

利用了来自SARS-CoV-1和SARS-CoV-2感染的RNA测序数据.
使用circRNA识别工具 (circRNA_finder,find_circ,CIRI2) 进行检测.
通过使用CirRNAPL.PL进行circRNA识别和表征.
进行了目标基因预测和功能丰富分析.

主要成果:

在SARS-CoV-1中确定了151个circRNA,在SARS-CoV-2中确定了470个circRNA,这表明SARS-CoV-2的编码能力更高.
观察到只有少数病毒circRNAs表现出高表达水平,正链产生更多的circRNAs.
发现病毒circRNAs和癌症之间的关联,表明它在调节宿主细胞功能中的作用.

结论:

SARS-CoV-1/2编码具有独特特征和功能的circRNAs.
病毒circRNAs与癌症和宿主细胞调节有关.
这项研究为了解冠状病毒circRNA机制提供了基础资源.

关键词:

在SARS-CoV-1/2中.标志性特征的描述.表达式分析,表达式分析.功能功能功能功能功能标识标识标识标识标识

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