SP1激活的USP27X-AS1通过USP7介导的AKT稳定促进肝细胞癌的进展
Chen Su1,2, Haoquan Zhang1,2, Jie Mo1,2
1Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
Clinical and translational medicine
|January 27, 2024
概括
肝细胞癌 (HCC) 是由致癌的长非编码RNA (lncRNA) USP27X-AS1.1.恶化. 这种lncRNA通过稳定AKT促进HCC的进展,为HCC治疗提供了一个潜在的新点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 肝细胞癌 (HCC) 仍然是癌症相关死亡的主要原因.
- 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在癌症发展中的作用.
- 在HCC病变发生过程中,lncRNAs的特定功能尚未完全被理解.
研究的目的:
- 研究新型长非编码RNAUSP27X-AS1在肝细胞癌 (HCC) 中的作用.
- 阐明USP27X-AS1影响HCC进展的分子机制.
- 评估USP27X-AS1作为HCC的潜在生物标志物和治疗标.
主要方法:
- 在使用生物信息学和qRT-PCR的HCC患者中评估USP27X-AS1表达.
- 进行了功能实验,以确定USP27X-AS1在HCC扩散和转移中的作用.
- 利用RNA拉下,RNA免疫沉降 (RIP) 和CUT-RUN测试来发现分子机制和调节因素.
主要成果:
- 高USP27X-AS1表达与HCC患者的预后不佳相关.
- USP27X-AS1通过促进USP7介导的AKT二维基因化和稳定,促进HCC的扩散和转移.
- 转录因子SP1被确定为USP27X-AS1转录的关键激活剂.
结论:
- 致癌性lncRNAUSP27X-AS1通过USP7-AKT通路驱动HCC的进展.
- 通过SP1激活USP27X-AS1的转录有助于HCC的发展.
- USP27X-AS1代表了一种有前途的预测生物标志物和肝细胞癌的治疗标.
相关概念视频
PI3K/mTOR/AKT Signaling Pathway
3.6K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.6K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
The JAK-STAT Signaling Pathway
8.9K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.9K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Interactions Between Signaling Pathways
6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
Anaphase Promoting Complex
2.9K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.9K


