母亲的高脂肪饮食影响卵子的含量,并导致梅达卡鱼的异常发育
Yusuke Inoue1, Manatsu Fukushima1, Go Hirasawa1,2
1Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan.
Endocrinology
|January 27, 2024
概括
麦达卡鱼中的母体高脂肪饮食导致后代变形. 卵子的变化包括氨基酸分解减少和脂质增加,影响胚胎发育.
科学领域:
- 发展生物学 发展生物学
- 营养科学 营养科学
- 生殖生物学 生殖生物学
背景情况:
- 母亲的营养显著影响后代的发育和代谢健康.
- 卵细胞组成 (有机体,RNA,代谢物) 是孕产妇饮食效应的关键媒介.
- 了解这些影响是复杂的,特别是在有胎盘和牛奶贡献的哺乳动物中.
研究的目的:
- 研究母亲高脂肪饮食 (mHFD) 对梅达卡鱼后代发育的影响.
- 为了识别由mHFD引起的成熟卵中的变化.
- 阐明母亲营养对胚胎发育的影响背后的机制.
主要方法:
- 麦达卡鱼被食高脂肪饮食,以评估对后代的影响.
- 分析了胚胎形和基因表达 (转录,翻译).
- 对成熟的卵进行了转录和代谢分析.
- 研究了女性卵细胞缩和肝脏基因表达.
主要成果:
- 母亲的高脂肪饮食增加了后代的胚胎形.
- 在后代中观察到的转录和翻译相关基因的下调.
- 成熟的蛋显示氨基酸/葡萄糖代谢降低,脂质升高,和中度的内等质网膜应激.
- 接受mHFD治疗的女性表现出卵细胞衰减的增加和卵蛋白基因表达的减少.
结论:
- 减弱的氨基酸代谢和高水平的卵脂是mHFD诱导后代变形的主要原因.
- 母亲的高脂肪饮食改变了卵子的组成,影响了胚胎的发育.
- 这项研究提供了对非哺乳动物脊椎动物父母营养效应的见解.
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