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在中对Enterococcus cecorum特异性抗体的血清监测

Amanda Silberborth¹, Jana Schnug¹, Silke Rautenschlein¹

¹Clinic for Poultry, University of Veterinary Medicine Hannover, Foundation, Buenteweg 17, 30559 Hannover, Germany.

Veterinary immunology and immunopathology

|January 27, 2024

概括

一种新的间接ELISA测定方法可以有效地检测的Enterococcus cecorum (EC) 感染,比传统方法识别更多的阳性病例. 虽然对实验研究有希望,但对于可靠的育种群体监测需要进一步改进.

科学领域:

兽医免疫学兽医免疫学
细菌学细菌学是一门学科.
禽类的健康禽类的健康

背景情况:

致病性Enterococcus cecorum (EC) 在肉类中引起显著的骨感染.
有效的干预策略是有限的,强调需要预防措施,如疫苗接种.
缺乏关于的EC感染或疫苗接种反应的血清学数据.

研究的目的:

开发和验证一个EC特定的间接ELISA用于的血清监测.
评估ELISA在实验和现场环境中检测EC感染的实用性.
在感染或接种疫苗后评估EC特异性免疫球蛋白Y (IgY) 血清转化.

主要方法:

使用EC抗原检测IgY的间接ELISA的开发.
来自实验性和自然感染EC的的血清样本的分析.
将ELISA结果与实时PCR和细菌学培养物的比较.
疫苗接种和未接种肉类繁殖群的监测.

主要成果:

开发的ELISA显示出与PCR和培养方法相当或实质性的协议.
与PCR/培养相比,ELISA检测到感染的EC阳性样本比例较高.
实验性感染的EC特定IgY水平在化42天后达到峰值.
在接种疫苗和未接种疫苗的育种群体之间,没有发现IgY水平的显著差异.
在未接种疫苗的繁殖群中,ELISA阳性样本的持续增加被注意到,这表明潜在的交叉反应性.

结论:

新开发的间接ELISA是实验性研究中EC的血清学监测的合适工具.
该试验在检测EC阳性中显示出比目前的诊断方法更高的灵敏度.
需要进一步优化,可能使用重组抗原,以减少背景和提高育种群监测的特异性.

关键词:

抗体是一种抗体.艾丽莎·伊丽莎·伊丽莎虫虫 (Enterococcus cecorum) 是一种虫,它可以生长在水中.肉类类型的.血清学监测是指进行血清学监测.

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相关概念视频

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...