在临床实验室中LC-MS/MS的演变
Songlin Yu1, Yutong Zou1, Xiaoli Ma1
1Department of Laboratory Medicine, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Science, Beijing, China.
Clinica chimica acta; international journal of clinical chemistry
|January 27, 2024
概括
液体染色学-并联质谱学 (LC-MS/MS) 提供了临床优势,但在生物标志物选择,自动化和标准化方面面临挑战. 通过公用事业驱动的方法和协作努力解决这些问题将加强其临床整合.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 生物标志物发现发现
背景情况:
- 液体染色学-双重质谱学 (LC-MS/MS) 越来越多地被认为具有临床实用性.
- 目前,由于分析物选择,自动化和标准化方面的挑战,广泛采用目前受到限制.
研究的目的:
- 审查目前阻碍LC-MS/MS临床应用的挑战.
- 提出潜在的决议,以克服这些局限性,并促进更广泛的采用.
主要方法:
- 关于LC-MS/MS应用和临床实践中的挑战的综合文献综述.
- 对当前局限性的分析,包括分析物选择,自动化,参考间隔和质量控制.
- 讨论拟议的解决方案,重点关注以实用驱动的生物标志物选择,方法创新,标准化和质量保证.
主要成果:
- 确定的主要挑战包括不适当的生物标志物选择,不充分的自动化,缺乏标准化的参考间隔,以及不充分的质量控制计划.
- 拟议的决议强调优先考虑生物标志物选择的实际实用性,开发高通量自动化,建立多中心参考间隔,并创建商业质量控制材料.
- 协调和标准化工作对于临床LC-MS/MS至关重要,预计商业套件和LDT将共存.
结论:
- 解决分析物选择,自动化和标准化方面的挑战对于LC-MS/MS的临床整合至关重要.
- 未来的进步将集中在简化,自动化,智能化和标准化上,增强临床医生和技术人员的日常使用.
- 专业人员之间的协作努力对于LC-MS/MS的成功临床实施至关重要.
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