核化调节卵巢反应通过FSH受体在毛囊发育期间的卵巢反应
Tiantong Wang1, Zhiwei Zhang2, Changduo Qu3
1Department of Thoracic Surgery, Cancer Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China; College of Basic Medical Sciences, Dalian Medical University, 9 West Section Lvshun South Road, Dalian, Liaoning 116044, China.
Journal of advanced research
|January 27, 2024
概括
由FUT8调节的核化对女性生育至关重要,它通过确保适当的卵泡刺激激素受体 (FSHR) 功能和卵细胞发育来确保女性生育. 减少的fucosylation会损害FSHR信号,导致卵巢反应不佳.
科学领域:
- 生殖生物学 生殖生物学
- 葡萄糖生物学 葡萄糖生物学
- 分子内分泌学分子内分泌学
背景情况:
- 卵巢对卵泡刺激激素 (FSH) 的低反应导致不孕.
- FSH受体 (FSHR) 的N-糖化是关键的,核化由基转移酶8 (FUT8) 是一个关键的修改.
- 改变的核化会影响各种生理过程,需要对其在女性生育能力中的作用进行研究.
研究的目的:
- 调查FUT8介导核化在调节FSHR功能的作用及其对女性生育能力的影响.
- 确定核化在卵细胞和卵泡发育中的重要性.
主要方法:
- 使用莱克斑和实时PCR分析患者样本 (反应较差).
- 产生Fut8基因淘汰赛小鼠和FUT8淘汰赛人类颗粒细胞系 (KGN-KD).
- 在体外受精 (IVF) 试验的应用,西部涂抹,分子相互作用研究,免疫光和免疫沉.
主要成果:
- 核心化损失显著损害了FSHR与FSH的结合和粒状细胞下游信号传递.
- 缺少FUT8导致对卵细胞成熟和植入前发育至关重要的下调的转录.
- 减少的FUT8会影响花质细胞-卵细胞相互作用,跨区域投射形成和卵细胞发育能力,这些能力可以通过L-fucose的管理来部分恢复.
结论:
- 核化是女性生育的关键调节者,通过FSH/FSHR通路起作用.
- 减少FSHR化与患者的卵巢反应 (POR) 较差有关.
- 通过FUT8介导的核心化对于哺乳动物卵细胞和卵泡发育至关重要.
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