Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Reversible and irreversible BTK inhibition promotes stem-like functional multipotency, persistence, and antitumor efficacy of CD19-directed CAR T cells in mantle cell lymphoma.

Leukemia·2026
Same author

1-Palmitoyl-4-piperidinopiperidine exhibits anticancer effects in murine triple-negative breast cancer EO771 cells associated with STAT3 signaling suppression.

Naunyn-Schmiedeberg's archives of pharmacology·2026
Same author

Circadian Dependence of Subacute Renal Toxicity of Cadmium.

Journal of applied toxicology : JAT·2026
Same author

Gradual Occlusion of an Anterior Choroidal Artery Aneurysm Using Overlapping Flow Diversion and Short-term Anticoagulation: A Case Report.

NMC case report journal·2026
Same author

Flow Diverter Stents for Recurrent Intracranial Aneurysms after Stent-Assisted Coil Embolization: Safety, Efficacy, and Technical Considerations-A Comprehensive Review.

Journal of neuroendovascular therapy·2026
Same author

Genetic variants associated with Sjögren's disease subtypes stratified by clinical feature.

Frontiers in immunology·2026

相关实验视频

Updated: Jul 4, 2025

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
07:13

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes

Published on: February 13, 2021

2.2K

单细胞多组学解码了小鼠二次部发育的监管程序.

Fangfang Yan1, Akiko Suzuki2,3,4, Chihiro Iwaya2,3

  • 1Center for Precision Health, School of Biomedical Informatics, The University of Texas Health Science Center at Houston, Houston, TX, 77030, USA.

Nature communications
|January 27, 2024
PubMed
概括

palatogenesis 中的基因调节中断会导致口腔裂口. 这项研究绘制了小鼠口腔发育中的表观遗传和基因表达变化,确定了前后口腔形成的关键转录因子SHOX2和MEOX2.

更多相关视频

Live Imaging of Mouse Secondary Palate Fusion
06:10

Live Imaging of Mouse Secondary Palate Fusion

Published on: July 27, 2017

7.6K
Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice
06:56

Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice

Published on: January 21, 2021

4.5K

相关实验视频

Last Updated: Jul 4, 2025

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
07:13

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes

Published on: February 13, 2021

2.2K
Live Imaging of Mouse Secondary Palate Fusion
06:10

Live Imaging of Mouse Secondary Palate Fusion

Published on: July 27, 2017

7.6K
Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice
06:56

Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice

Published on: January 21, 2021

4.5K

科学领域:

  • 发展生物学 发展生物学
  • 基因组学就是基因组学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 口腔裂是一种常见的先天性出生缺陷.
  • palatogenesis 期间的基因调节中断与它的发展有关.

研究的目的:

  • 为了全面地映射小鼠二次口腔发育过程中的表观遗传和转录动态.
  • 为了确定调节形成的关键转录因子.

主要方法:

  • 单细胞多基因组测序 (scMultiome) 在36154个来自小鼠二级的细胞上进行,跨越胚胎日E12.5-E14.5.5.
  • 染色体可访问性和基因表达在单个细胞内同时进行了分析.
  • 计算分析,包括轨迹推断和in silico扰动,用于识别监管关系.

主要成果:

  • 构建了从神经衍生的细胞到独特的 palatal 谱系的五个分化轨迹.
  • 确定血统的转录因子的特征是将开放的染色体信号与基因表达联系起来.
  • 鉴定出SHOX2和MEOX2分别是前和后发育的关键调节者.

结论:

  • 这项研究提供了一个详细的地图表,介绍了口腔发育过程中的表观遗传和转录变化.
  • 这些发现提供了对口腔发育背后的分子机制的宝贵见解,并确定了口腔裂的潜在治疗点.
  • 生成的数据集将成为未来对先天性出生缺陷研究的关键资源.