开发染色学方法以根据多种维生素的可溶性和极性来确定多种维生素的配方:使用三个绿色评估工具进行比较研究
Sara Abdel Basset Galal1, Eman Saad Elzanfaly2, Emad Mohamed Hussien1
1Egyptian Drug Authority, 51 Wezaret El- Zeraa Street, Agouza, Giza, 12618, Egypt.
BMC chemistry
|January 27, 2024
概括
开发了两种高性能液体色谱法,用于量化骨质疏松症治疗中的伊普里夫拉,甲酸,铁素,维生素D3和素. 这些经过验证的方法确保药物配方中药物分析的准确性.
科学领域:
- 分析化学 分析化学
- 药品分析 药品分析
背景情况:
- 骨质疏松症的治疗通常涉及复杂的药物组合.
- 准确量化药物活性成分对于药物的有效性和安全性至关重要.
研究的目的:
- 开发和验证两种互补的高性能液态染色学 (HPLC) 方法,用于确定骨质疏松辅助剂配方中的五种关键化合物.
- 确保这些方法适用于散装药物分析和制药剂量形式.
主要方法:
- 方法A:逆相HPLC与紫外线检测 (254纳米) 使用Agilent Eclipse XDB-C18列和渐变移动相 (氨酸缓冲:甲醇) 对于ipriflavone,酸,pyridoxine和维生素D3.
- 方法B:使用Exsil 100 SCX柱和极性化合物氨酸的特定移动相进行强酸交换 (SCX) 染色学与紫外线检测 (210 nm).
- 这两种方法的验证都按照ICH指南Q2 (R1) 进行.
主要成果:
- 方法A成功分离和量化了易普里夫拉,亚斯科布酸,氧素和维生素D3.
- 方法B有效地分离了氨酸,没有衍生,克服了在逆相柱上高极性的挑战.
- 这两种方法都证明了在药物配方中分析目标化合物的选择性和有效性.
结论:
- 开发的HPLC方法为特定的骨质疏松症辅助剂配方的质量控制提供了可靠和选择性的分析工具.
- 这两种方法的互补性解决了药物成分的多样化物理化学性质所带来的分析挑战.
- 使用ESA,GAPI和AGREE进行的绿色评估表明,开发的分析程序的环境性能令人满意.
关键词:
亚斯科伯酸是什么? 亚斯科伯酸是一种绿色的评估 绿色的评估伊普里夫拉 (ipriflavone) 是一种一种氨酸 HCl 是一种氨酸.皮里多克素 HCl 的含量.在RP-HPLC方法中使用RP-HPLC方法.维生素D3 维生素D3 是一种维生素.更多相关视频
10:22Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties Lactuca sativa L. and Crop Wild Relatives Lactuca spp.
Published on: June 30, 2020
13.5K
06:14Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique
Published on: October 6, 2022
1.8K
相关概念视频
Analyte Adsorption and Distribution
662
In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
662
Chromatography: Introduction
4.3K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
4.3K
Chromatographic Methods: Classification
2.3K
Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
Chromatographic techniques are typically named by...
2.3K
Principles Of Column Chromatography
6.8K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
6.8K
Chromatographic Methods: Terminology
2.2K
Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
2.2K
Extraction: Advanced Methods
447
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
447
