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Intronless Pabpc6编码了一个丸特定的细胞质多A结合蛋白,但对于小鼠†的精子生成是不可或缺的

Yuko Kaku¹, Yuka Isono¹, Hideto Tanaka¹

¹Institute of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.

Biology of reproduction

|January 28, 2024

概括

丸特异性多A结合蛋白6 (PABPC6) 结合mRNA,但对于小鼠的精子发生是不必要的. 在胚胎细胞中,PABPC6与其他多A结合蛋白具有功能冗余性.

科学领域:

分子和细胞生物学分子和细胞生物学
生殖生物学生殖生物学
遗传学遗传学是一个

背景情况:

聚A结合蛋白家族在mRNA调节中起着至关重要的作用.
众所周知,多个聚A结合蛋白家族成员,包括PABPC1,PABPC2/PABPt和PABPC1L/ePAB,都存在于小鼠丸中.
在基分析中,最近发现了一种新的丸特异性成员Pabpc6.

研究的目的:

描述新发现的丸特异性聚甲结合蛋白6 (PABPC6) 的表达,功能和定位.
通过分析缺乏PABPC6的小鼠,研究PABPC6在男性生殖细胞发育和精子生成中的作用.

主要方法:

免疫组织化学和西式斑点测试以确定丸细胞中PABPC6表达模式.
生物化学试验评估PABPC6的mRNA结合能力和与翻译因子的相互作用.
对精子体内PABPC6局部化的分析,特别是与多体和染色体的关联.
对PABPC6淘汰赛小鼠进行表型分析,以评估对蛋白质合成和精子生成的影响.

主要成果:

检测到PABPC6的表达在帕奇丁精子细胞的细胞质中,在圆形精子细胞中增加,在延长的精子细胞中减少.
PABPC6证明了多甲尾结合和与转化因子 (如EIF4G,PAIP1和PAIP2) 的相互作用.
与PABPC1不同,PABPC6与多体体的关联很小,并且在圆形精子体的染色体中得到丰富.
PABPC6淘汰赛小鼠表现出正常的丸蛋白合成和精子生成,这表明PABPC6没有重要的作用.

结论:

PABPC6是一种丸特定的多A结合蛋白,具有独特的局部化特征,与PABPC1.1不同.
尽管它具有mRNA结合能力和与翻译因子的相互作用,但PABPC6对于小鼠的精子生成并不重要.
在PABPC6突变小鼠中缺乏表型表明在雄性生殖细胞发育过程中与其他PABPC蛋白质的功能冗余.

关键词:

在PABPC6中,没有内置基因的基因.聚A结合蛋白质是多A结合蛋白质的组成部分.精子的产生是精子的产生.

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