计算机断层扫描对照剂对辐射诱导的基因表达和双链断裂的影响
Simone Schüle1,2, Felix Bunert1, Carsten Hackenbroch2,3
1Bundeswehr Institute of Radiobiology affiliated to the University of Ulm, Neuherbergstraße 11, 80937 Munich, Germany.
Radiation research
|January 28, 2024
概括
含的对比剂不会影响辐射诱导的基因表达或血液样本中的双链断裂. 这一发现支持在急性辐射综合征 (ARS) 患者中使用这些生物测量方法,这些患者需要增强对比度的CT扫描.
科学领域:
- 医学辐射物理学 医学辐射物理学
- 分子生物学分子生物学
- 生物医学成像技术 生物医学成像技术
背景情况:
- 来自核场景的联合伤害,如急性辐射综合征 (ARS) 与创伤,可能需要对比度增强的计算机断层扫描 (CT) 扫描.
- 类对比剂通常用于CT扫描,但它们对辐射诱导生物标志物的影响尚未完全理解.
研究的目的:
- 研究含的对比剂对辐射诱导的基因表达 (GE) 和双链断裂 (DSB) 的作用.
- 为了确定这些药物是否会干扰生物测量和ARS严重程度预测方法.
主要方法:
- 来自10名健康捐献者的全血样本用有或没有含有的对比剂的X射线 (0,1和4 Gy) 进行辐射.
- 用定量实时聚合酶连锁反应8小时后测量基因表达.
- 使用20分钟和8小时后的γH2AX + 53BP1免疫染量化双链断裂焦点.
主要成果:
- 含的对比剂没有显著改变辐射诱导的基因表达变化.
- 双链断裂焦点的数量没有受到对比剂的存在的影响.
- 观察到的轻微显著差异归因于个体间的变化,而不是对比剂.
结论:
- 含的对比剂不会干扰辐射诱导的基因表达或双链断裂诱导.
- 生物测量方法,包括基因表达和DSB测定,仍然可用于评估需要对比度增强CT扫描的患者的辐射暴露.
- 这些发现支持对比度增强CT在管理联合伤害方面的诊断效用.
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