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多重效应因子选用于在小麦原生质中使用积极防御记者识别内源性耐药基因

Salome Wilson¹, Bayantes Dagvadorj¹, Rita Tam¹

¹Research School of Biology, The Australian National University, Canberra, ACT, 2601, Australia.

The New phytologist

|January 28, 2024

概括

一种新的小麦原质细胞检测法使得植物病原体相互作用的高通量选成为可能. 这种工具加速了对抗性基因的识别,有助于开发抗病作物.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
农作物科学农作物科学

背景情况:

植物耐药性 (R) 和病原体激烈性 (Avr) 基因相互作用对于作物的疾病耐药性至关重要.
目前农作物的分子选工具不如模型生物的工具先进,阻碍了对农业重要R基因的识别.

研究的目的:

开发一种新的小麦原生质细胞测定方法,以高效大规模选Avr/R相互作用.
促进新型抗性基因的识别,加快研究植物防御信号的研究.

主要方法:

开发了一种小麦原质细胞试验,用于选Avr/R相互作用,而不需要R基因过度表达.
在多达50名Avr候选人的多重查中利用了多重查.
创建了一个基于Avr/R诱导的防御基因的促进子-露西法酶记者,与双色比度记者系统相结合.
包含一个自我复制的等离子体,以减少测试试剂的要求.

主要成果:

该试验可以有效地选大规模的Avr/R相互作用.
它允许访问广泛的R基因,包括尚未克隆的基因.
该系统对实验变异性和细胞死亡的读数进行正常化,提高了可靠性.
该试验表明,Avr候选查的吞吐量增加了.

结论:

开发的小麦原生质细胞检测方法显著提高了选Avr/R相互作用的效率.
这种工具加速了对抗性基因的识别和对植物防御机制的理解.
预计该试验将加速对小麦病原体的Avr识别,改善作物育种和疾病监测.

关键词:

这是一种狂热的狂热,一种激烈的狂热.防御可诱导的推动者效应器效应器是一个效应器.光酶是一种光酶.工厂防御系统的工厂防御系统耐药性基因的基因是耐药性的基因.小麦原塑是一种小麦原塑.

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