超灵敏的电化学发光传感器利用聚合诱导排放活性探测器在米粒中准确量化化物
Ming-Ming Chen1, Hang Gao2, Zhan-Biao Ge3
1Centre for Agriculture and Health, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing 210095, China.
Journal of agricultural and food chemistry
|January 29, 2024
概括
一个新的电化学发光生物传感器使用AIE-Pdots来敏感地检测大米中的无机化物 (As(III)). 该方法为食品安全和对污染环境监测提供了可靠的工具.
科学领域:
- 环境科学 环境科学
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- (As) 对生态系统和公共健康构成重大风险.
- 准确量化大米中无机化物 (As) 的含量对于食品安全至关重要.
研究的目的:
- 开发一种敏感和特定的电化学发光 (ECL) 生物传感器,用于在米中检测酸.
- 为了利用聚合诱导发射 (AIE) 活跃的Pdots来增强信号生成.
主要方法:
- 基于四乙烯的AIE活性Pdots的合成.
- 构建一个ECL共振能量传输 (ECL-RET) 系统,使用Pdots作为捐赠者和BHQ作为接受者.
- 开发一个As(III) 特定的aptamer测定,用于信号检测.
主要成果:
- 生物传感器显示了线性检测范围为10 pM至500 nM的As(III).
- 实现了5.8 pM (0.4 ppt) 的超低检测极限 (LOD).
- 传感器显示出高灵敏度和特异性,结果与实际大米样本中的HPLC-ICP-MS一致.
结论:
- 开发的基于AIE-Pdot的ECL生物传感器提供了一种敏感且可靠的方法,用于在中检测酸.
- 这项技术在改善食品安全,农业实践和对的环境监测方面具有重大潜力.
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