迪奥斯基宁生物合成途径及其在Dioscorea cirrhosa L中的调节
PeerJ
|January 29, 2024
概括
迪奥斯科雷亚罗萨结核含有13种迪奥斯基宁代谢产物. 研究人员确定了参与迪奥斯基宁生物合成的关键酶和转录因子,为这种药用植物提出了一条途径.
科学领域:
- 植物生物化学和分子生物学
- 代谢学和转录学.
- 药用植物的研究研究.
背景情况:
- Dioscorea cirrhosa tuber是一种传统的药用植物,具有潜在的药理学化合物.
- 之前,D. cirrhosa中diosgenin的存在和生物合成是不确定的.
研究的目的:
- 确认在D. cirrhosa tuber.中存在迪奥斯基宁及其代谢物.
- 为了阐明D. cirrhosa.中的diosgenin的生物合成途径.
- 为了确定关键的酶,转录因子和CYP450基因参与迪奥斯基因生物合成.
主要方法:
- 高性能液体染色学-并联质谱学 (HPLC-MS/MS) 用于代谢物识别.
- 转录组数据分析以确定参与生物合成的独基因和酶.
- 生物信息分析包括基因注释,遗传学分析和相关性分析.
主要成果:
- 在D. cirrhosa tuber.中发现了13种二氧化物代谢物.
- 建立了用于迪奥斯基宁生物合成的拟议途径,涉及21个关键酶和43个单基因.
- 22个转录因子被发现与迪奥斯基宁生物合成基因有很强的关联.
- 鉴定出了206个CYP450基因,其中有40个潜在的候选基因与迪奥斯基宁生物合成中的固醇修饰有关.
结论:
- 这项研究证实了迪奥斯基宁的存在,并阐明了它在D. cirrhosa中的生物合成途径.
- 确定了关键的调节基因,包括转录因子和CYP450s,为基因工程提供了目标.
- 这些发现为通过未来的研究来增强植物中的迪奥斯基宁生产提供了基础.
更多相关视频
07:59A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
9.8K
12:10Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster
Published on: April 14, 2017
10.5K
相关概念视频
Circadian Rhythms and Gene Regulation
4.1K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.1K
Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
IP3/DAG Signaling Pathway
12.1K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
12.1K
Regulation of Hormone Secretion
3.4K
Regulation of hormone secretion is a finely tuned orchestration driven by various types of stimuli, encompassing neural, humoral, and hormonal signals. Environmental cues instigate neural stimuli, where action potentials traverse nerve fibers to reach their designated targets. An illustrative scenario is the body's response to stress, wherein the sympathetic nervous system releases epinephrine from the adrenal glands, inducing the well-known 'fight or flight' reaction.
Humoral...
Humoral...
3.4K
