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马卢斯的完整的叶绿体基因组序列 × adstringens 扎贝尔"Hopa" (科)

Leiming Dong¹, Ruizhen Wang¹, Hengxing Liu¹

¹Key Laboratory of National Forestry and Grassland Administration on Plant Ex situ Conservation, Beijing Floriculture Engineering Technology Research Centre, Beijing Botanical Garden, Beijing, China.

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概括

装饰性的完整的叶绿体基因组

科学领域:

植物基因组学植物基因组学
人类遗传学是一个学科.
装饰园艺装饰园艺装饰园艺

背景情况:

装饰果品种"Hopa" (Malus × adstringens Zabel) 是因为它的美学品质而受到重视.
了解马卢斯属内的遗传关系对于繁殖和保护工作至关重要.

研究的目的:

组装和表征马卢斯"Hopa"的完整的叶绿体基因组.
为了阐明马卢斯"Hopa"在马卢斯属内的遗传学位置.
评估叶绿体基因组数据对于解决果品种关系的有用性.

主要方法:

采用下一代测序技术对整个叶绿体基因组进行测序.
用于基因组组装,基因注释和SSR识别的生物信息分析.
基因组学分析基于叶绿体基因组数据.

主要成果:

组装了Malus"Hopa"的完整的质体基因组,测量160,230bp,GC含量为36.50%.
该基因组包括128个基因,包括蛋白质编码,tRNA和rRNA基因,并包含73个简单序列重复 (SSR).
遗传学分析表明,Malus 'Hopa'与Malus spectabilis和Malus sieversii.密切相关.

结论:

叶绿体基因组序列为Malus 'Hopa'提供了一个宝贵的资源.
хлоропласт基因组数据有效地解决了果品种之间的家族遗传关系.
这项研究证明了使用马卢斯的cp基因组数据识别母系血统的可行性.

关键词:

马卢斯 (Malus) 是一个不良的东西.叶绿体基因组的基因组鱼果 (Crabapple) 是一个生殖系关系的关系.

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