基于RNA干扰的外源双链RNA给尼古提纳烟草 (Nicotiana tabacum) 赋予了对Rhizoctonia solani AG-3的耐药性
Yan Wang1, Yi Guo1, Shiping Guo2
1Liaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Pest management science
|January 29, 2024
概括
宿主诱导的基因沉默 (HIGS) 和外源的dsRNA有效抑制了由Rhizoctonia solani引起的烟草点疾病. 准EndPGs和RPMK1基因为疾病控制提供了一个新的RNAi策略.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 烟草菌 (Rhizoctonia solani) 在全球范围内造成烟草点病的重大损失.
- 目前对这种疾病的控制策略和耐药品种有限.
- 宿主诱导的基因沉默 (HIGS) 和外源的dsRNA显示出抑制疾病的潜力.
研究的目的:
- 研究HIGS和外源dsRNA在控制Rhizoctonia solani.中的有效性.
- 确定参与R. solani病变和生长的关键基因.
- 为基于RNAi的疾病管理策略提供基础.
主要方法:
- 基于TRV的HIGS被用于使尼古提纳塔米亚纳的EndPGs或RPMK1基因沉默.
- 在体外实验中,R. solani hyphae被用标记的dsRNA治疗.
- 测试了dsRNA向endoPGs,RPMK1和组合序列 (dsRNA-PGMK) 的应用.
- 使用定量实时PCR来确认基因表达变化.
主要成果:
- 通过HIGS抑制endoPGs或RPMK1显著减少了烟草点病.
- dsRNA治疗 (dsRNA-endoPGs,dsRNA-RPMK1,dsRNA-PGMK) 在体外抑制了R. solani的叶生长.
- 外源的dsRNA应用抑制了植物叶子中的疾病进展.
- 定量实时PCR在dSRNA治疗后证实了endoPGs和RPMK1的减少表达.
结论:
- 通过RNAi方法准endoPGs和RPMK1基因是针对R. solani.的一个可行的策略.
- 这些发现为预防和控制由R. solani引起的疾病提供了新的方向.
- 这项研究为开发针对R. solani感染的基于RNAi的解决方案提供了理论基础.
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