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用生物信息学辅助提取所有PCa微RNA及其向基因

Akilandeswari Ramu¹, Jayaprakash Chinnappan¹

¹Anthropology and Health Informatics Lab, Department of Bioinformatics, Bharathiar University, Coimbatore, Tamil Nadu, India.

MicroRNA (Shariqah, United Arab Emirates)

|January 29, 2024

概括

这项研究通过对文献数据的计算分析来识别和分类前列腺癌 (PCa) 微RNA (miRNA) 和它们的标. 关键的miRNA和AKT1和TP53等目标基因被突出显示为潜在的PCa疗法.

科学领域:

在瘤学瘤学.
遗传学是一个遗传学.
生物信息学是一种生物信息学.

背景情况:

微RNA (miRNA) 是基因表达的关键调节者,影响细胞生长和分化等过程.
毫米RNAs的失调与各种癌症的发展和进展有关,包括前列腺癌 (PCa).
了解miRNAs在PCa中的特定作用对于开发向疗法至关重要.

研究的目的:

从现有的文献中全面识别和分类前列腺癌相关的microRNAs (PCa miRNAs).
阐明PCa miRNAs与它们的基因之间的功能关系.
根据miRNA-基因相互作用来确定前列腺癌的潜在治疗点.

主要方法:

在PubMed数据库的文献挖掘,直到2021年12月为PCa相关的文章.
使用Pubmed.mineR包进行microRNA提取和手动修复以进行分类.
使用生物信息学工具,包括STRING,PANTHER,ToppGene,ShinyGO,MCODE,cytoHubba和Cytoscape用于网络和途径分析.

主要成果:

检索和分类了605种独特的瘤反转基因,120种瘤抑制性miRNA和24种与PCa相关的治疗miRNA.
鉴定了分类PCamiRNAs的重要途径,功能和枢纽基因.
为PCa.构建了一个全面的基因-miRNA网络.

结论:

包括miR-27a,miR-34b和miR-143/-145在内的几种miRNA,以及AKT1,SRC和TP53等目标基因,已被确定为前列腺癌中的重要基因.
这些发现为了解PCa中miRNA介导的基因调节提供了基础.
鉴定的miRNAs和标有可能成为前列腺癌治疗的未来治疗策略.

关键词:

这是一本"Corpus Corpus".生物信息学.生物信息学.微RNAs 是一个微型RNA.在委员会上.系统生物学系统生物学瘤抑制剂是一种瘤抑制剂.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...