相关实验视频

Updated: Jul 4, 2025

Controlled Cortical Impact Model for Traumatic Brain Injury

Controlled Cortical Impact Model for Traumatic Brain Injury

Published on: August 5, 2014

创伤性脑损伤引起的昼夜时钟的破坏

Lu-Ting Kuo^1,2, Hsueh-Yi Lu³, Yi-Hsing Chen⁴

¹Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, 7 Chun-Shan South Road, Taipei, 100, Taiwan. kuoluting@gmail.com.

Journal of molecular medicine (Berlin, Germany)

|January 29, 2024

概括

创伤性脑损伤 (TBI) 破坏昼夜节律. 这项研究发现,大鼠的急性体内血瘤改变了血液细胞中关键昼夜基因 (Per2,Cry1,Bmal1) 的节律表达,并破坏了体温调节.

科学领域:

神经科学是一个神经科学.
时间生物学时间生物学
分子生物学分子生物学

背景情况:

在创伤性脑损伤 (TBI) 后,循环节律障碍很常见.
在TBI之后,周围血液白细胞 (PBL) 中昼夜基因的表达仍然未被调查.
急性亚皮血瘤 (ASDH) 是一种严重的TBI形式.

研究的目的:

在患有ASDH的老鼠的PBL中研究昼夜基因的mRNA表达.
评估ASDH对特定昼夜基因 (Per1, Per2, Per3, Cry1, Cry2, Bmal1, Clock) 节律表达的影响.
在ASDH之后评估体温节律的变化.

主要方法:

在48小时内从假操作和ASDH大鼠的PBL中量化昼夜基因mRNA表达.
在两组中,两天内每4小时测量直肠温度.
坐标分析用于估计基因表达和体温的中位数,节奏,振幅和形.

主要成果:

对Per2,Cry1和Bmal1mRNAs的节奏表达在假操作的老鼠的PBL中观察到.
ASDH大鼠在Per2,Cry1和Bmal1mRNA表达中表现出被破坏的节律性.
与模拟操作的老鼠相比,ASDH老鼠表现出受损的直肠温度节奏,幅度减小和移转.

结论:

大鼠的ASDH导致PBLs中特定的昼夜基因的表达失调.
与ASDH相关的TBI显著改变了体温节律.
需要进一步的研究来阐明TBI后扰乱的昼夜网络的病理生理学.

关键词:

循环节的基因是循环节的基因.在Cosinor分析中,温度温度是指温度.创伤性脑损伤是一种创伤性脑损伤.

更多相关视频

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

Published on: January 24, 2013

The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression

The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression

Published on: July 24, 2018

相关概念视频

Circadian Rhythms and Gene Regulation

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...