在Cyclocarya paliurus中与三烯和黄类生物合成相关的基因的功能性表征
Shuang-Yan Zhang1,2, Yu-Qing Peng1,2, Gui-Sheng Xiang1,2
1College of Agronomy and Biotechnology, National-Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwest, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Fengyuan Road, Panlong District, Kunming, 650201, Yunnan, China.
Planta
|January 29, 2024
概括
研究人员在Cyclocarya paliurus中确定了三类和黄类生物合成的关键酶. 这项研究揭示了氧化二烯循环酶和UDP-glycosyltransferases参与生产潜在药物应用的有价值化合物.
科学领域:
- 植物生物化学 植物生物化学
- 分子生物学分子生物学
- 代谢学 代谢学 代谢学
背景情况:
- 赛克洛卡利亚帕利乌鲁斯 (Cyclocarya paliurus) 是一种罕见的中国植物,富含生物活性三聚烯酸盐和黄酸糖化物.
- 这些化合物对高血压,高脂血症和糖尿病具有潜在的药用益处.
- 它们在C. paliurus中的生物合成的分子途径尚不清楚.
研究的目的:
- 鉴定和描述参与C. paliurus中三类桑因和黄类糖化物生物合成的关键酶.
- 阐明这些有价值的化合物的产生背后的分子机制.
- 为未来的合成战略提供基础.
主要方法:
- 用转录组和代谢组分析来研究基因表达和化合物概况.
- 参与三二生物合成的八种氧化二二烯循环酶 (OSC) 的功能特征.
- 两种黄酸尿二酸盐 (UDP) - 葡萄糖转移酶 (UGTs) 的解,催化黄酸葡萄糖合成的最后步骤.
主要成果:
- 八个OSC的功能性特征,特定的酶被确定用于催化氧化素转化为各种三类骨架 (例如,3-epicabraleadiol,dammarenediol-II,β-amyrin,cycloartenol,lanosterol).
- CpalOSC1没有显示可检测的催化活性.
- 两个UGTs,CpalUGT015和CpalUGT100,被确定为关键的最后的糖化步骤在黄类糖化物生物合成.
结论:
- 这项研究成功地确定了关键的OSC和UGT,它们负责C. paliurus中三烯酸盐和类糖化物的生物合成.
- 这些发现揭示了这些途径的关键基因.
- 这些已识别的酶为未来生物技术生产有价值的三烯酸盐和黄类糖化物提供了潜力.
相关概念视频
01:18Aromatic Hydrocarbon Cations: Structural Overview
2.8K
Cycloheptatriene is a neutral monocyclic unsaturated hydrocarbon that consists of an odd number of carbon atoms and an intervening sp3 carbon in the ring. The three double bonds in the ring correspond to 6 π electrons, which is a Huckel number, and therefore satisfies the criteria of 4n + 2 π electrons. However, the intervening sp3 carbon disrupts the continuous overlap of p orbitals. As a result, cycloheptatriene is not aromatic.
Removing one hydrogen from the intervening CH2 group...
Removing one hydrogen from the intervening CH2 group...
2.8K
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
02:19Morphogenesis
28.2K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
28.2K
01:18Frost Circles for Different Conjugated Systems
2.7K
The inscribed polygon method is consistent with Hückel’s 4n + 2 rule and helps to learn whether the given cyclic compound is aromatic or not. The compound is stable and aromatic if every bonding molecular orbital (MO) is completely filled with a pair of electrons. However, if the non-bonding or antibonding orbitals are filled with electrons, the compound is unstable and not aromatic. Consider the Frost circle diagrams for cycloalkenes containing 4 to 8 carbons.
2.7K
01:27C4 Pathway and CAM
45.5K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
45.5K
02:22Introduction to Plant Diversity
44.7K
From Water to Land
44.7K