在酸压力下对Vibrio parahaemolyticus进行转录组分析
Yilin Lin1, Meimei Fang1, Jun Liu2
1South China University of Technology, School of Food Sciences and Engineering, Guangzhou, 510640, China.
Applied microbiology and biotechnology
|January 29, 2024
概括
乳酸 (PLA) 通过破坏营养吸收,细胞代谢和生物膜形成,有效地抑制Vibrio parahaemolyticus. 这种天然有机酸为控制食品工业中的细菌病原体提供了一个有希望的战略.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
背景情况:
- 乳酸 (PLA) 是一种天然的有机酸,具有已知的抗微生物特性.
- 活型血栓溶解菌 (Vp) 是一种重要的食物传播病原体.
- 了解PLA对Vp的抗菌机制对于食品安全至关重要.
研究的目的:
- 为了研究PLA对Vibrio parahaemolyticus ATCC17802 (Vp17802) 的抗菌机制.
- 在转录基层探索PLA对Vp17802的影响.
- 为在细菌病原体控制中使用PLA提供理论基础.
主要方法:
- 最低抑制度 (MIC) 的确定和时间杀伤分析.
- 使用RNA测序进行转录基因分析.
- 分析与新陈代谢,营养物质运输和毒性因素相关的基因表达.
主要成果:
- PLA (MIC 3.2 mg/mL) 通过破坏细菌膜并降低ATP水平来抑制Vp17802的生长.
- RNA测序确定了1616个差异表达的基因,其中icd2基因的下调影响TCA循环,氨酸生物合成和氧化酸化.
- 通过阻断载体,PLA会扰乱营养的获取,并干扰生物膜的形成,定数感应和运动.
结论:
- 通过破坏营养吸收,细胞代谢 (icd2基因) 和生物膜形成,PLA抑制Vp17802.
- 通过影响运动性和重要的代谢途径,PLA减少了Vp17802的毒性.
- 酸呈现出一种可行的天然化合物,用于控制食品中的Vp污染.
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