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Plant Tissue Culture

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

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Updated: Jul 4, 2025

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

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使用临时浸泡系统进行兰花微繁殖:一篇评论

Obdulia Baltazar-Bernal¹, Evelia Guadalupe Mora-González², Marco A Ramírez-Mosqueda³

¹Laboratório de Cultivo de Tejidos Vegetales, Colegio de Postgraduados Campus Cordoba, Amatlan de los Reyes, Veracruz, Mexico. obduliabb@colpos.mx.

Methods in molecular biology (Clifton, N.J.)

|January 29, 2024

概括

临时浸泡系统 (TIS) 提供了一种有效的兰花微型传播方法,通过定期将扩展物暴露在营养介质和空气中. 本综述分析了TIS在兰花繁殖中的应用,涵盖了关键因素和适应效应.

关键词:

沉浸频率是沉浸的频率.大规模生产的大规模生产.兰花科 (Orchidaceae) 是一个植物.PLBs 是一个PLB.野生兰花是一种野生兰花.

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Development of Microfluidic Devices to Study the Elongation Capability of Tip-growing Plant Cells in Extremely Small Spaces

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Published on: May 22, 2018

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Last Updated: Jul 4, 2025

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

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Published on: May 22, 2018

科学领域:

植物生物技术植物生物技术
园艺科学园艺科学
植物组织培养.

背景情况:

兰花微型繁殖在实现高效和可扩展的幼苗生产方面面临着挑战.
临时浸泡系统 (TIS) 为传统静态培养方法提供了一个有希望的替代方案.

研究的目的:

审查和综合TIS在兰花繁殖中的当前应用.
确定影响TIS在兰花微型繁殖中的成功的关键因素.

主要方法:

对53篇关于TIS用于兰花繁殖的研究论文进行系统审查.
基于探索剂类型,培养介质成分和TIS生物反应器设计的研究分析.
评估浸泡频率,持续时间及其对幼苗发育和适应的影响.

主要成果:

通过交替浸入营养物质和暴露在空气中,TIS增强了兰花的繁殖,防止水浸.
最佳的TIS参数 (频率,浸泡时间) 取决于所使用的兰花种和剂.
TIS对微繁殖兰花的适应阶段产生积极影响.

结论:

临时浸泡系统是提高兰花微型繁殖效率的宝贵工具.
进一步研究优化TIS参数对各种兰花物种的进一步研究是有必要的.
TIS促进了更好的适应,可能减少了体外培养中的损失.