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基于人类多能干细胞的测试,以预测发育毒性.

Madhura Banerjee1, Aritrika Das1, Prarthana Chatterjee1

  • 1School of BioSciences and Technology, Vellore Institute of Technology, Vellore, India.

Methods in molecular biology (Clifton, N.J.)
|January 29, 2024
PubMed
概括

环境中的毒素会损害生殖健康和后代的发育. 人类多能干细胞为发育毒性试验提供了经济有效,道德的替代方案,提高了预测准确度.

关键词:
分化细胞是分化的细胞.不同化的差异化人类诱导的多能干细胞干细胞.人类多能干细胞是人类多能干细胞.试验化合物 试验化合物

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科学领域:

  • 毒理学 毒理学 毒理学
  • 发展生物学 发展生物学
  • 干细胞研究 干细胞研究

背景情况:

  • 人类接触环境毒素会影响生殖健康和后代发育,影响神经,心脏和肌肉骨系统.
  • 出生缺陷的患病率不断上升,需要改进检测发育毒性的方法.
  • 使用动物模型进行常规毒性测定是昂贵的,在伦理上存在问题,并且由于物种间的变化,具有有限的预测价值.

研究的目的:

  • 使用人类多能干细胞预测化合物发育毒性的方法的概述.
  • 概述一种高效和有效的程序,用于对发育性毒素进行高通量查.
  • 突出人类多能干细胞在毒性测试中的优势.

主要方法:

  • 利用人类多能干细胞,包括诱导多能干细胞,进行体外发育毒性测定.
  • 开发和介绍一项简化协议,用于同时选多种化合物.
  • 专注于利用人类起源和多能干细胞可获得性的测试.

主要成果:

  • 人类多能干细胞为动物模型的发育毒性测试提供了可行的替代方案.
  • 概述的程序可以同时选多种化合物,提高效率.
  • 这种方法提供了一种更具预测性和道德合理的方法来评估发育性毒性.

结论:

  • 人类多能干细胞是体外发育毒性试验的优秀选择,因为它们的可访问性,人类起源和伦理优势.
  • 提出的方法提供了一种具有成本效益和高度预测性的方法,用于查发育性毒素.
  • 实施这些测试可以显著减少与有毒物质暴露有关的出生缺陷的发生.