Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Cell-free MSC secretome therapy ameliorates diabetic testicular injury via antioxidant mechanisms.

Journal of molecular histology·2026
Same author

520 nm and 660 nm light-emitting diodes modulates pancreatic development and beta cell functions in zebrafish embryos.

Photochemistry and photobiology·2024
Same author

Comparison of the cytotoxicity and zebrafish embryo toxicity of insect repellent ingredients: p-Menthane-3,8-diol synthesized by green chemistry from <i>Eucalyptus citriodora</i> and N,N-diethyl-meta-toluamide.

Drug and chemical toxicology·2024
Same author

Diethylhexyl phthalate exposure amplifies oxidant and inflammatory response in fetal hyperglycemia model predisposing insulin resistance in zebrafish embryos.

Toxicology and industrial health·2024
Same author

Assessment of Developmental Neurotoxicity Using Semi-automatic Behavior Analysis System for Zebrafish.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Alcian Blue Staining for Chondrocranium Development in Zebrafish.

Methods in molecular biology (Clifton, N.J.)·2024

相关实验视频

Updated: Jul 4, 2025

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

11.7K

微阵列分析以确定斑马鱼胚胎中的基因表达变化.

İsmail Ünal1, Merih Beler1, Derya Cansız2

  • 1Department of Biochemistry, Institute of Health Sciences, Marmara University, Istanbul, Turkey.

Methods in molecular biology (Clifton, N.J.)
|January 29, 2024
PubMed
概括

斑马鱼胚胎是研究化学暴露影响的有价值模型. 微阵列分析,使用RNA完整数 (RIN) 值,有助于管理这些透明,快速发展的模型中的基因表达研究工作量.

关键词:
微阵列分析分析RIN 值的使用情况.一个斑马鱼胚胎.在cRNARNA中.

更多相关视频

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

10.9K
High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

22.2K

相关实验视频

Last Updated: Jul 4, 2025

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

11.7K
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

10.9K
High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

22.2K

科学领域:

  • 发展生物学 发展生物学
  • 毒理学 毒理学 毒理学
  • 遗传学 是一个遗传学.

背景情况:

  • 暴露于化学物质会影响人类从胚胎到胎儿的发育.
  • 斑马鱼是研究的强大的模型生物,由于基因组同质性,小尺寸和高繁殖率.
  • 斑马鱼胚胎提供了诸如子宫外发育和透明度等优势,增加了它们的研究实用性.

研究的目的:

  • 描述微阵列分析作为斑马鱼胚胎中基因表达决定的关键技术.
  • 突出技术如何减轻复杂遗传研究中的工作负担.
  • 解释RNA完整数 (RIN) 在微阵列测定中的重要性.

主要方法:

  • 微阵列分析用于基因表达造型.
  • 评估RNA完整数 (RIN) 以评估RNA质量 (1-10级).
  • 使用斑马鱼胚胎作为模型系统.

主要成果:

  • 微阵列分析是对斑马鱼胚胎基因表达研究的主要技术.
  • 对于决定微阵列实验的可行性,RIN值至关重要.
  • 这项技术有助于管理大规模的基因表达数据.

结论:

  • 以RIN值为指导的微阵列分析对于在斑马鱼胚胎中有效的基因表达研究至关重要.
  • 斑马鱼胚胎为毒理学和发育研究提供了一个强大的模型.
  • 像微阵列这样的技术进步显著提高了发育毒理学的研究能力.