相关实验视频

Updated: Jul 4, 2025

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

向G9a/DNMT1甲基转移酶活性阻碍了在肝细胞瘤中IGF2介导的生存

Salih Demir¹, Negin Razizadeh¹, Emilie Indersie²

¹Department of Pediatric Surgery, Dr. von Hauner Children's Hospital, LMU University Hospital, LMU Munich, Germany.

Hepatology communications

|January 29, 2024

概括

用CM-272准UHRF1显示出通过逆转表观遗传变化来治疗肝母细胞瘤的前景. 这种方法,特别是结合西斯,为高风险患者提供了一种新的策略.

科学领域:

在瘤学瘤学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
药理学药理学是指药理学的学科.

背景情况:

肝细胞母细胞瘤 (HB) 患者的治疗结果各不相同,这表明除了遗传之外的因素,如表观遗传变化,影响治疗反应.
UHRF1 (像PHD和戒指域1一样的ubiquitin) 是一个关键的表观遗传调节器,与HB有关,使其成为潜在的治疗标.

研究的目的:

为了研究CM-272的疗效,一种双重抑制UHRF1-依赖的DNA和基因素甲基化,在治疗肝细胞瘤.
阐明CM-272抗癌作用的分子机制及其作为HB治疗剂的潜力.

主要方法:

利用患者衍生异种移植 (PDX) 模型 (体外和体内) 来评估CM-272的药物反应.
采用RNA测序和西部斑点来了解CM-272处理的机制基础.

主要成果:

UHRF1在HB中高度表达,与预后不佳有关.
CM-272选择性地降低了HB细胞活力,降低了IGF2生存途径的调节,并诱导了亡.
在临床前的PDX模型中,CM-272与西斯普拉丁具有协同效应,并且在临床前的PDX模型中显示出有效性,尽管有副作用.

结论:

抑制UHRF1相关的表观遗传修饰,如IGF2介导的存活率,是高风险HB的可行策略.
将CM-272与西斯普拉丁结合在一起,为肝细胞母细胞瘤治疗提供了一个有前途的治疗方法.

更多相关视频

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Published on: January 7, 2019

相关概念视频

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...