相关实验视频
Updated: Jul 4, 2025

12:31
In Vivo Modeling of the Morbid Human Genome using Danio rerio
Published on: August 24, 2013
20.7K
来自进化的计算表观模型的体内功能表型
Sophia Alvarez1, Charisse M Nartey1, Nicholas Mercado1
1Department of Biological Sciences, University of Texas at Dallas, Richardson, TX 75080.
概括
计算模型现在可以进化具有增强功能的蛋白质. 我们的新算法SEEC使用自然蛋白家族数据来创建更活跃的变体,推进进化生物学和生物医学应用.
科学领域:
- 进化生物学是进化的生物学.
- 计算生物学是一种计算生物学.
- 生物化学 生物化学
背景情况:
- 进化的计算模型对于理解序列变异,系系和进化途径至关重要.
- 验证模型输出的体内功能对于准确的进化算法至关重要.
- 突变之间的相互作用 (epistasis) 在蛋白质进化中起着关键作用.
研究的目的:
- 为了证明从自然蛋白质家族推断出的表观作用的力量,以演化功能蛋白质变体.
- 引入和验证一种新的算法,即带有表观贡献的序列进化 (SEEC).
- 评估进化的蛋白质变体的体内功能和活性.
主要方法:
- 开发了SEEC算法,结合了从天然蛋白质家族推断出的表皮质.
- 使用联合序列概率的哈密尔顿式作为健身指标.
- 在体内实验测试了进化的大肠杆菌TEM-1变体的β-乳糖酶活性.
主要成果:
- 在SEEC进化中,蛋白质变体具有数十种突变,同时保留了必要的催化和相互作用部位.
- 进化的变种保留了类似家族的功能,并且比野生类型表现出更高的活动.
- 不同的表达式推断方法模拟了不同的选择强度,较弱的选择回顾了中性进化.
结论:
- 通过自然的表观作用,SEEC有效地进化了具有增强活性的功能性蛋白质变体.
- 该算法可以模拟不同的进化动态,包括中性进化.
- 东南欧经济区在新功能化,病毒适应性景观表征和疫苗开发方面具有应用潜力.
相关概念视频
Epistasis
46.8K
In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
46.8K
Epistasis Analysis
5.0K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
5.0K
Background and Environment Affect Phenotype
6.5K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.5K
Genetic Screens
4.9K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
4.9K
Incomplete Dominance
22.6K
Gregor Mendel's work (1822 - 1884) was primarily focused on pea plants. Through his initial experiments, he determined that every gene in a diploid cell has two variants called alleles inherited from each parent. He suggested that amongst these two alleles, one allele is dominant in character and the other recessive. The combination of alleles determines the phenotype of a gene in an organism.
22.6K
In-vitro Mutagenesis
13.9K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
13.9K

