唾液微生物组在体外暴露下对时间,环境和表面特征的反应
Litao Huang1, Xiaomin Liang1, Guichao Xiao1
1Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China.
Forensic science international. Genetics
|January 29, 2024
概括
唾液污点中的微生物群随着时间和环境的变化而发生变化. 这项研究开发了模型来识别唾液,评估污染,估计沉积时间,帮助法医科学.
科学领域:
- 法医微生物学 法医微生物学
- 环境微生物学环境微生物学
- 微生物生态学 微生物生态学
背景情况:
- 唾液污点是有价值的法医和生态证据.
- 随着时间的推移和环境的变化,唾液污点中的微生物动力学尚未得到充分研究.
研究的目的:
- 在各种条件下分析唾液污点微生物群.
- 开发唾液识别和沉积后时间 (TsD) 估计的模型.
主要方法:
- 暴露于不同条件 (时间,载体,环境) 的唾液样本的高通量测序.
- 微生物组成分析,Mfuzz聚类和中性模型预测.
- 随机森林 (RF) 和XGBoost分类/回归模型的开发.
主要成果:
- 唾液菌群 (例如,链球菌) 短期稳定;环境细菌 (例如,伪菌) 长期入侵.
- 湿度促进了环境植物的生长;户外环境显示了随机的社区集会.
- 分类模型达到>98%的准确性;回归模型以低误差估计了TSD.
结论:
- 唾液污点微生物组的组成随着时间的推移和环境暴露而可预测地发生变化.
- 开发了用于法医应用的准确模型:唾液识别,污染评估和TSD推断.
- 这些发现有助于更好地了解沉积生物样本中的微生物动力学.
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