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A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

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Published on: October 21, 2010

关于针对Candida auris殖民性的查方案的建议

S E Leonhard¹, G M Chong¹, D E Foudraine¹

¹Department of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, Rotterdam, The Netherlands.

The Journal of hospital infection

|January 29, 2024

概括

一个新的聚合酶连锁反应 (PCR) 查协议有效地识别了住院患者的Candida auris殖民. 这种方法有助于控制这种多药耐药酵母在医疗机构的传播.

关键词:

这是一种Candida Candida.菌类的耐药性是真菌的耐药性.医院医院医院医院分子诊断技术分子诊断技术

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科学领域:

医学真菌学医学真菌学
传染性疾病传染性疾病
临床微生物学临床微生物学

背景情况:

黄是一种多药耐药的酵母菌,会导致严重的医院感染.
它的全球传播令人担忧,生物膜使持续的殖民和环境污染成为可能.
通过标准培养来识别C. auris的困难给感染控制带来了挑战.

研究的目的:

开发和验证一种快速查协议,用于发现C. auris殖民.
将内部聚合酶链反应 (PCR) 与优化的培养方法相结合,以实现准确的检测.

主要方法:

开发了C. auris特定的原料和针对ITS区域的探针.
使用12个C. auris分离物和103个其他Candida物种分离物进行验证的PCR测定,达到100%的准确性.
确定该试验的检测极限大约为每PCR四个细胞.

主要成果:

对高风险患者的查协议于2023年2月实施.
在8个月内,199名患者进行了查,确定了7例阳性病例 (4%的患病率).
该协议涉及从多个身体部位收集抽样.

结论:

开发的基于PCR的查协议显示了在医院环境中控制C. auris的前景.
早期识别C. auris殖民可能有助于及时实施感染控制措施.