胃肠道中逐层自组装的粉基纳米囊的结构变化:对它们的M细胞向输送和运输效率的影响
Lei Xie1, Yiping Zhang1, Ling Chen1
1Ministry of Education Engineering Research Center of Starch and Protein Processing, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
针对M细胞的新纳米囊显示改善了口服输送. 这些RGD植入的纳米适应胃肠道pH值的变化,提高卵白蛋白的运输效率.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 药物输送系统 药物输送系统
背景情况:
- 在胃肠道 (GIT) 中细胞向传递载体的结构变化的特征对于理解它们的有效性至关重要.
- 优化输送和运输效率,特别是针对M细胞的向,仍然是口服药物输送的挑战.
研究的目的:
- 为了制造和表征RGD移植的碳素甲基粉 (CMS) 和阴离子四级粉 (QAS) 纳米囊,用于增强M细胞向.
- 调查GIT的pH和酶变异对纳米囊结构,载体相互作用和卵蛋白 (OVA) 释放行为的影响.
主要方法:
- 使用CMS和QAS制造五重层纳米囊,装有OVA.
- 在不同pH和酶条件下分析纳米囊结构变化,泽塔潜力和OVA释放.
- 利用异热定位热度计和分子动态模拟来研究载体相互作用和pH效应.
主要成果:
- 纳米囊尺寸从229.2nm增加到479.8nm,并且在口服输送期间,泽塔电位从-1.08mV降至-33.33mV,表明结构放松.
- pH值的变化显著影响了载体之间的静电相互作用,pH值的增加降低了亲和常数.
- 大约84.42%的OVA成功地传送到M细胞,运输效率是Caco-2细胞的五倍.
结论:
- 通过调节静电相互作用,RGD植入的纳米显示出适应GIT pH变化的能力.
- 开发的纳米囊系统显示出改善口服输送和M细胞向效率的巨大潜力.
- 这项研究为先进的纳米载体设计为有针对性的口服药物输送铺平了道路.
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