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在Polygonatum kingianum Coll.中的碳水化合物的鉴定. 并且可以抑制氧化应激

Junyuan Huang¹, Yanlan Chen¹, Yi Su¹

¹South China Agricultural University, College of Food Science, Guangzhou 510642, China.

International journal of biological macromolecules

|January 29, 2024

概括

来自Polygonatum kingianum (PKP-1) 的新型果糖被描述并评估其抗氧化特性. 通过提高关键的抗氧化酶和谷氨水平,PKP-1在减轻肝脏氧化压力的显著潜力.

科学领域:

碳水化合物化学碳水化合物化学
自然产品化学自然产品化学
生物化学生物化学

背景情况:

从Polygonatum物种,特别是Polygonatum kingianum Coll. 的多糖化合物的结构阐明. 而Hemsl,仍然是有限的.
多糖因其多样化的生物活动而闻名,包括抗氧化性质.

研究的目的:

从Polygonatum kingianum Coll.中提取,净化和表征一种胰岛素类型的果糖素 (PKP-1). 在Hemsl.et.
评估纯化PKP-1的体外抗氧化活性,重点关注其对肝脏氧化应激的影响.

主要方法:

在Polygonatum kingianum中提取和净化PKP-1.
通过核磁共振 (NMR) 等技术确定分子量,单糖化合物组成和糖链接.
在体外抗氧化剂测定中,使用暴露于自由脂肪酸的HepG2细胞,测量超氧化物脱酶 (SOD) 和催化酶 (CAT) 的酶活性,以及谷氨 (GSH) 水平.

主要成果:

鉴定PKP-1是一种胰岛素类型的果实素,分子量为4.802kDa.
聚糖由银糖,葡萄糖和果糖 (0.8%:7.2%:92.0%) 组成,具有特定的链接结构 (→1-β-d-Fruf-2→2,6-β-d-Fruf-1→).
通过增加SOD,CAT活动和HepG2细胞中的GSH水平,PKP-1显著降低了肝氧化应激.

结论:

具有特征的果糖PKP-1具有显著的抗氧化特性.
PKP-1 显示了缓解自由脂肪酸诱导的肝氧化应激的潜力.
波利格纳 (Polygonatum kingianum) 是一种新型天然抗氧化剂的潜在来源.

关键词:

糖酸结合物糖酸结合物糖酸结合物植物植物植物聚合物聚合物聚合物

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