溶液对粉结构的影响:从凝化原生玉米粉中分离烯胺分离物的新方法
Xinxin Lv1, Yan Hong2, Zhengbiao Gu2
1Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Wuxi 214122, Jiangsu Province, People's Republic of China; School of Food Science and Technology, Jiangnan University, 1800 LiHu Avenue, Wuxi 214122, Jiangsu Proevince, People's Republic of China.
Carbohydrate polymers
|January 29, 2024
概括
化 (CaCl2) 提供了一种有效,环保的方法来溶解玉米粉,提取粉和粉素 (AP),结构影响最小. 这种新的方法产生了高AP提取率,在粉分成中呈现出有前途的进步.
科学领域:
- 碳水化合物化学 碳水化合物化学
- 生物聚合物科学 生物聚合物科学
- 绿色化学 绿色化学
背景情况:
- 粉分成粉和粉素 (AP) 需要完全溶解粉而不会降解.
- 开发高效和环保的溶剂对于推进粉分离技术至关重要.
研究的目的:
- 为了比较二甲基硫氧化物 (DMSO),氧化物 (NaOH) 和化 (CaCl2) 溶液在玉米粉分离中的有效性.
- 评估这些溶剂对粉结构和AP产品参数的影响.
- 为了确定一个最佳的溶剂高产AP提取.
主要方法:
- 用DMSO,NaOH和CaCl2溶液在50°C时溶解玉米粉.
- 分析了粉解构效应,结构变化 (晶体和分子),以及AP产品参数.
- 对于每个溶剂,可溶性,再生和AP提取率都被量化.
主要成果:
- 在50°C时,CaCl2溶液实现了高玉米粉溶解度 (98.96%),促进了均的颗粒再生.
- 所有测试的溶剂都改变了玉米粉晶体结构,但没有衍生.
- 在玉米粉的分子结构上,CaCl2对玉米粉的分子结构的影响最小.
- 使用CaCl2溶液的AP提取率达到了69.45%.
结论:
- CaCl2 是一种高效且极少侵入性的溶剂,用于玉米粉溶解和AP提取.
- 这项研究提出了一种新,高效和环保的方法,通过粉分化提取AP.
- 这些发现支持CaCl2作为与DMSO和NaOH相比的粉分离的优质溶剂.
更多相关视频
相关概念视频
01:27SDS-PAGE
28.0K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
28.0K
02:35DNA Agarose Gel Electrophoresis
96.7K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
96.7K