相关实验视频

Updated: Jul 4, 2025

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Published on: July 20, 2016

通过贝利斯·希尔曼 (Baylis Hillman) 引述的位置无定位位特异性蛋白质生物结合

Mudassir H Mir¹, Sangeeta Parmar¹, Chhaya Singh¹

¹Department of Chemistry, Indian Institute of Science Education and Research (IISER) Bhopal, Bhopal Bypass Road, Bhauri, Bhopal, 462066, Madhya Pradesh, India.

Nature communications

|January 29, 2024

概括

我们开发了一种新的方法,贝利斯·希尔曼编排的蛋白质氨基乙醇标签 (BHoPAL),用于使用小分子进行特定位置的蛋白质标签. 这种技术使稳定的生物结合和双重标记能够用于化学生物学和药物开发中的各种应用.

科学领域:

化学生物学化学生物学
有机化学有机化学
生物化学生物化学

背景情况:

特定位置的蛋白质标签对于化学生物学和药物开发至关重要.
N-终端囊蛋白 (N-Cys) 的1,2-氨基乙醇部分为区域选择性蛋白质标记提供了独特的反应性.

研究的目的:

报告稳定的蛋白质生物结合的超快速Z选择性反应.
开发一个无定位的蛋白质标签技术.
为了使双重标记蛋白质生物结合物的生成.

主要方法:

利用伊沙衍生的贝利斯希尔曼附加物和1,2-氨基乙醇进行新型反应,形成双 heterocyclic 脚手架.
开发了贝利斯·希尔曼编排的蛋白质氨基乙醇标签 (BHoPAL) 用于蛋白质生物结合.
采用基于脂酸结合酶的技术,在所需的蛋白质部位引入1,2-氨基乙醇部分,实现位置无关标签.

主要成果:

在体外和活细胞条件下使用BHoPAL实现了稳定的蛋白质生物结合.
通过在任何所需位置引入1,2-aminothiol部分来证明无定位的蛋白质标签.
生成的双标记蛋白质生物结合物,在单个蛋白质分子的特定部位上具有不同的标签.

结论:

披露的蛋白质生物结合工具包有助于生成单个和多个标签的蛋白质小分子生物结合物.
应用包括生物物理测试,细胞成像和治疗性蛋白质药物合物生产.
这种新型双异环基架在合成和药物化学中有潜在的应用.

更多相关视频

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition

Published on: December 23, 2016

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

相关概念视频

Tagging and Fusion Proteins

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

Ligand Binding and Linkage

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...